介质细胞干细胞不朽化协议的元分析:再生医学指南
Priscila Elias Ferreira Stricker1, Nathalia Barth de Oliveira1, Bassam Felipe Mogharbel1
1Pelé Pequeno Príncipe Research Institute Research & Pequeno Príncipe Faculties, Curitiba, Brazil.
Current stem cell research & therapy
|January 15, 2024
概括
本综述确定了将介质干细胞 (MSC) 永久化常见方法. 使用hTERT和SV40T基因的病毒转染分别在人类和动物MSC中最常见.
科学领域:
- 干细胞生物学 干细胞生物学
- 再生医学是一种再生医学.
- 细胞工程 细胞工程
背景情况:
- 介质细胞干细胞 (MSCs) 对再生医学至关重要.
- 对MSC的不朽化是其治疗应用的关键.
- 需要MSC永生化的标准化方法.
研究的目的:
- 系统地审查和描述常见的方法来使人类和动物介质干细胞 (MSCs) 不朽化.
- 识别MSC永生化中经常使用的基因和技术.
- 为了评估用于永久化MSCs的验证试验.
主要方法:
- 使用关键词"介酶干细胞"和"不朽化"进行系统的文献搜索.
- 搜索的数据库包括SCOPUS,PUBMED和科学直接 (2000-2022年).
- 接下来是PRISMA指导方针,在选后包括了39篇文章.
主要成果:
- 病毒转感染是最常见的不朽化方法.
- 人类MSCs在28项研究中得到了永恒化,动物MSCs在11.
- 在人类和动物MSC中,分别使用hTERT和SV40T基因最频繁.
结论:
- 病毒传染是MSC永生化的一个普遍方法.
- 虽然瘤发生性试验并非普遍进行,但其他验证方法,如qRT-PCR和扩散试验是常见的.
- 在验证试验中进一步标准化可能是有益的.
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