Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Proteomics01:33

Proteomics

7.3K
A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term...
7.3K
Ribosome Profiling02:24

Ribosome Profiling

3.5K
Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
3.5K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Inhibition of the Atypical Kinase WNK1 as a Therapeutic Strategy in TAL-related T-cell Acute Lymphoblastic Leukemia.

Blood·2026
Same author

HOOK2 downregulation compromises the tumorigenic and stemness properties of ovarian cancer cells by increasing endoplasmic reticulum stress.

Cell death & disease·2026
Same author

Discovery of cerebrospinal fluid biomarkers for different dementias using mass spectrometry-based proteomics.

Alzheimer's & dementia (Amsterdam, Netherlands)·2026
Same author

Phosphoproteomics of Hypertrophic Cardiomyopathy Patient Myocardium and Novel hiPSC-CM Model Reveal Protein Kinase A as a Modulator of Microtubule Repolymerization.

bioRxiv : the preprint server for biology·2026
Same author

Reduced Versus Oxidized NAD<sup>+</sup> Precursors Drive Distinct Transcriptomic, Proteomic, and Metabolic Profiles in Hepatocytes.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology·2026
Same author

Boosting the Speed and Accuracy of Protein Quantification Algorithms in Mass Spectrometry-Based Proteomics.

Journal of proteome research·2026
Same journal

Identification of Novel Interacting Proteins of FUZ and GPR161.

Proteomics·2026
Same journal

Light-Induced Proteomic Changes in Pseudomonas aeruginosa Biofilms.

Proteomics·2026
Same journal

Decade-Resolved Proteomic Profiling of Gastric Cancer FFPE Archives: Evaluating Storage-Associated Shifts and Signal Stability Over 50 Years.

Proteomics·2026
Same journal

Proteome-Scale Mining of Metal-Associated Proteins of Monkeypox Virus.

Proteomics·2026
Same journal

Optimized Sample Handling Minimizes Peptide Adsorption to Plastics to Enable High Sensitivity Evosep Based Chemical Proteomics.

Proteomics·2026
Same journal

Toward Predicting Pandemic Potential: A Comparative Analysis of Virus-Host Interactions Between Diverse Influenza A Viruses and the Human Innate Immune System.

Proteomics·2026
查看所有相关文章

相关实验视频

Updated: Jul 5, 2025

Detection of Protein Ubiquitination Sites by Peptide Enrichment and Mass Spectrometry
11:54

Detection of Protein Ubiquitination Sites by Peptide Enrichment and Mass Spectrometry

Published on: March 23, 2020

9.5K

蛋白质组的秘密处理:一种方法比较方法.

Catarina Almeida-Marques1,2, Frank Rolfs1,2, Sander R Piersma1,2

  • 1Department Laboratory Medical Oncology, Amsterdam University Medical Center, Amsterdam, Netherlands.

Proteomics
|January 15, 2024
PubMed
概括
此摘要是机器生成的。

在溶液中消化 (ISD) 之后的乙沉是分析癌症分泌蛋白质的最佳方法. 这种方法增强了生物标志物发现的蛋白质识别和可重复性.

关键词:
生物标志物生物标志物在溶液中消化消化.蛋白质组学 蛋白质组学一些秘密一些秘密.一次性的一次性射击.

更多相关视频

Optimized Protocol for the Extraction of Proteins from the Human Mitral Valve
09:13

Optimized Protocol for the Extraction of Proteins from the Human Mitral Valve

Published on: June 14, 2017

12.9K
Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification
10:37

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification

Published on: November 15, 2017

12.0K

相关实验视频

Last Updated: Jul 5, 2025

Detection of Protein Ubiquitination Sites by Peptide Enrichment and Mass Spectrometry
11:54

Detection of Protein Ubiquitination Sites by Peptide Enrichment and Mass Spectrometry

Published on: March 23, 2020

9.5K
Optimized Protocol for the Extraction of Proteins from the Human Mitral Valve
09:13

Optimized Protocol for the Extraction of Proteins from the Human Mitral Valve

Published on: June 14, 2017

12.9K
Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification
10:37

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification

Published on: November 15, 2017

12.0K

科学领域:

  • 蛋白质组学是指蛋白质组学.
  • 癌症生物学 癌症生物学
  • 生物标志物发现发现

背景情况:

  • 癌细胞分泌体提供了有价值的生物标志物,因为它反映了瘤与环境的相互作用.
  • 生物流体中分泌蛋白的高检测能力使其成为诊断的有希望的.

研究的目的:

  • 评估六种不同的处理癌症分泌物样本的工作流程.
  • 通过使用一次性质谱测量来确定高通量秘密蛋白质组的最佳方法.

主要方法:

  • 测试了六种秘密体样本处理工作流程,涉及蛋白质度 (超过,乙沉,冷化) 和消化 (凝消化,溶液消化).
  • 评估工作流程是基于蛋白质识别号码,独特识别,可重复性和检测小功能蛋白 (细胞因子,化学因子,生长因子) 的数据.
  • 单射纳米液体色谱-并联质谱 (纳米LC-MS/MS) 用于分析.

主要成果:

  • 乙蛋白沉与溶液消化 (ISD) 结合,确定了最多的蛋白质 (2246).
  • 这种方法还显示出优异的可重复性,相关系数 (r) 为0.94%,变化系数 (CV) 为19%.
  • 在溶液消化工作流程中,特别是在降水时,可以更好地识别小的功能性重要蛋白质.

结论:

  • 乙蛋白沉与溶液消化相结合是高通量秘密蛋白质组的推方法.
  • 这种优化的工作流提高了癌症相关蛋白质的识别和量化,以发现生物标志物.