相关实验视频

Updated: Jul 5, 2025

Embryo Microinjection and Knockout Mutant Identification of CRISPR/Cas9 Genome-Edited Helicoverpa Armigera Hübner

Embryo Microinjection and Knockout Mutant Identification of CRISPR/Cas9 Genome-Edited Helicoverpa Armigera Hübner

Published on: July 1, 2021

植物中的CRISPR-Cas介导基因组编辑的3Bs:探索基础知识,生物信息学和生物安全景观

Lalit Kharbikar^1,2, Rocktotpal Konwarh^3,4, Monoswi Chakraborty^5,2

¹ICAR - National Institute of Biotic Stress Management (NIBSM), Raipur, India.

Physiology and molecular biology of plants : an international journal of functional plant biology

|January 15, 2024

概括

该CRISPR-Cas9系统提供先进的植物基因组编辑功能,使精确的DNA修改作物改进. 生物信息学工具和数据库对于推进CRISPR-Cas研究和应用至关重要.

科学领域:

植物基因组学植物基因组学
分子生物学分子生物学
生物技术是生物技术.

背景情况:

克里斯普尔-Cas9系统是精确修改植物基因组的革命性工具.
它利用像Cas9这样的RNA引导的DNA内核酶 (RGEN) 来诱导特定地点的DNA裂变.
修改后的Cas9变体,如死亡的Cas9 (dCas9),可以实现转录激活 (CRISPRa) 和干扰 (CRISPRi).

研究的目的:

提供关于CRISPR-Cas系统的基本原理和植物工作流程的概述.
突出生物信息学在推进CRISPR-Cas研究中的作用.
讨论关于CRISPR-Cas编辑植物的当前监管环境.

主要方法:

审查CRISPR-Cas系统的原则和应用在植物基因组编辑.
探索生物信息学数据库,算法和支持CRISPR-Cas研究的网络工具.
对有关CRISPR-Cas编辑作物的全球监管政策的分析.

主要成果:

在植物中,CRISPR-Cas9促进了DNA序列的修改,基因淘汰 (CRISPRko) 和基因调控 (CRISPRa/i).
生物信息学资源显著提高了CRISPR-Cas编辑的效率和适用性.
对CRISPR-Cas编辑植物的监管环境在国际上有所不同,这影响了技术采用.

结论:

克里斯普尔-卡斯技术为改善作物和农业创新提供了巨大的潜力.
综合生物信息学方法对于优化CRISPR-Cas工具开发和部署至关重要.
了解和导航监管环境对于基因组编辑在农业的广泛影响至关重要.

关键词:

生物安全生物安全在CRISPR中编辑编辑.克里斯普尔-Cas9是什么意思适应气候的农作物提高作物质量提高作物质量在DNA裂变的过程中,由RNA引导的DNA-内核酶.监管政策监管政策监管政策 dCas9 的使用情况

更多相关视频

Genome Editing in Mammalian Cell Lines using CRISPR-Cas

Genome Editing in Mammalian Cell Lines using CRISPR-Cas

Published on: April 11, 2019

Author Spotlight: Streamlining Rice Breeding with CRISPR/Cas for Obtaining Optimal Phenotypic and Agronomic Traits

Author Spotlight: Streamlining Rice Breeding with CRISPR/Cas for Obtaining Optimal Phenotypic and Agronomic Traits

Published on: January 3, 2025

相关概念视频

CRISPR and crRNAs

CRISPR and crRNAs

Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...

Homologous Recombination

Homologous Recombination

The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...

What is Genetic Engineering?

What is Genetic Engineering?

Plant Breeding and Biotechnology

Plant Breeding and Biotechnology

Crop cultivation has a long history in human civilization, with records showing the cultivation of cereal plants beginning at around 8000 BC. This early plant breeding was developed primarily to provide a steady supply of food.

Transgenic Plants

Transgenic Plants

Recombinant DNA technology called transgenesis is often used to add a foreign gene or remove a detrimental gene from an organism. Such genetically modified organisms are called transgenic organisms.
The first-ever transgenic plant was a tobacco plant developed in 1983 that showed resistance against the tobacco mosaic virus. Since then, many transgenic plants have been developed and commercialized for improving the agricultural, ornamental, and horticultural value of a crop plant. Transgenic...