基于生物信息学的发现脊椎间盘退化生物标志物和免疫炎症透物
Chao Song1, Daqian Zhou1, Kang Cheng1
1Department of Orthopedics and Traumatology (Trauma and Bone-setting), Laboratory of Integrated Chinese and Western Medicine for Orthopedic and Traumatic Diseases Prevention and Treatment The Affiliated Traditional Chinese Medicine Hospital of Southwest Medical University Luzhou Sichuan China.
JOR spine
|January 15, 2024
概括
这项研究确定了27个与椎间盘退化 (IVDD) 和免疫反应有关的枢纽基因. 细胞衰老和细胞亡途径是IVDD进展的关键驱动因素,提供了新的研究方向.
科学领域:
- 整形外科 整形外科 整形外科
- 生物信息学是一种生物信息学.
- 免疫学 免疫学 免疫学
背景情况:
- 椎间盘退化 (IVDD) 是一种普遍的骨科疾病,其分子机制尚未完全理解.
- 识别关键的分子参与者和免疫相互作用对于理解IVDD的发病过程至关重要.
研究的目的:
- 使用生物信息学方法发现IVDD的新生物标志物.
- 调查与IVDD相关的免疫炎症透.
主要方法:
- 使用了GeneCards,DisGeNet和GEO数据集 (GSE124272,GSE150408,GSE153761) 来进行基因收集.
- 进行了蛋白质-蛋白质相互作用网络分析 (STRING) 和功能丰富 (KEGG,GO).
- 检查了免疫细胞透和通过qPCR和西欧斑块验证的枢纽基因表达.
主要成果:
- 鉴定了27个不同表达的枢纽基因,这些基因与免疫反应,化学激素信号和炎症有关.
- 功能分析强调了细胞衰老,细胞亡和T细胞分化 (Th1,Th2,Th17) 的作用.
- 在IVDD患者中观察到T细胞,淋巴细胞,B细胞,NK细胞减少,单细胞,中性粒细胞,CD8T细胞增加. 在IVDD模型中验证了SP1,VEGFA,IL-6和CASP3表达的增加.
结论:
- 细胞衰老和亡信号通路,以及已识别的枢纽基因,在IVDD发育中至关重要.
- 这些发现为未来的研究提供了基础,重点是IVDD中的衰老途径.
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