在体内,通过微结构成像窗口进行无标签组织组织学
Claudio Conci1, Laura Sironi2, Emanuela Jacchetti1
1Department of Chemistry, Materials and Chemical Engineering "Giulio Natta," Politecnico di Milano, Piazza L. da Vinci 32, 20133 Milan, Italy.
APL bioengineering
|January 15, 2024
概括
这项研究引入了一种用于无标签显微镜的新型植入式装置,可对生物材料免疫反应进行纵向评估. 这种方法为生物材料开发提供了传统遗传病理学的转变性替代方案.
科学领域:
- 生物材料科学 生物材料科学
- 显微镜的使用方法
- 免疫学 免疫学 免疫学
背景情况:
- 生物材料评估的传统组织病理学是耗时和昂贵的,阻碍了纵向研究.
- 非线性兴奋显微镜提供了一个无标签的,体内替代品,有可能克服当前的局限性.
研究的目的:
- 开发和验证一种可植入的微结构装置,用于对生物材料的免疫反应进行无标签,非线性激发显微镜评估.
- 为细胞识别和量化创建组织学观察和无标签成像之间的相关性.
主要方法:
- 使用双光子激光聚合制造3D晶格微结构装置的制造.
- 装置植入胚胎蛋的胆 ?? 膜 (CAM) 中7天.
- 组织学分析 (H&E染色) 与非线性激发和共聚焦显微镜图像的比较,以构建细胞地图.
主要成果:
- 用无标签成像测量微观结构中招募的细胞的量化和粒细胞的识别.
- 通过产生第二和自光成像来识别原蛋白和微血管.
- 观察到的组织反应与CAM愈合一致,而不是异物反应.
结论:
- 开发的微结构装置能够使用非线性激发显微镜对组织-生物材料接口进行无标签的体内成像.
- 这种方法为纵向生物材料验证和生物工程提供了传统基因病理学的改造性替代方案.
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