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快速里埃图形定量相位显微镜用于体外无标签成像

Ruofei Wu¹, Zicong Luo¹, Mingdi Liu¹

¹School of Physics and Optoelectronic Engineering, Foshan University, Foshan 528000, China.

Biomedical optics express

|January 15, 2024

概括

这项研究引入了快速定量相位显微镜 (FQP-FPM),这是一种用于高通量实时成像的新方法. 它显著提高了生物医学研究中无标签活细胞分析的效率.

科学领域:

生物医学光学生物医学光学
定量阶段成像技术定量阶段成像技术
显微镜的使用方法

背景情况:

定量相显微镜 (QPM) 对于无标签成像至关重要,提供厚度和折射率量化.
里埃图形显微镜 (FPM) 提供高分辨率成像,但由于数据冗余和顺序获取,其效率较低.

研究的目的:

为实时,高通量成像开发更快的QPM方法.
克服传统FPM在活细胞应用中的成像效率方面的局限性.

主要方法:

开发基于富里埃图谱 (FQP-FPM) 的快速QPM,使用优化的环状下采样和并行采集.
与传统的FPM相比,大大减少了数据要求和算法代时间.
仅获得了十个原始图像,用于高通量定量阶段重建.

主要成果:

FQP-FPM实现了传统FPM数据和代时间的3.3%和4.4%,分别.
实现了高通量定量阶段重建,达到连贯衍射极限分辨率的三倍.
成功验证了体外无标签活细胞成像,观察细胞形态和亚细胞现象.

结论:

FQP-FPM为快速,高通量定量相位成像提供了重大进步.
该方法为实时体外无标签成像提供了先决条件.
以高分辨率和吞吐量观察动态细胞过程的潜在潜力.

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