功能化的光纤球形生物传感器,用于无标签,低极限检测IL-8蛋白质
Gyunel Rashidova1, Meruyert Tilegen1, Tri T Pham1
1Department of Biology, School of Sciences and Humanities, Nazarbayev University, Astana, 010000, Kazakhstan.
Biomedical optics express
|January 15, 2024
概括
这项研究引入了一种新的光纤生物传感器,用于检测唾液中介素-8 (IL-8) 蛋白. 这种非侵入性方法为唾液中疾病生物标志物检测提供了一种高度敏感且具有成本效益的方法.
科学领域:
- 生物医学工程 生物医学工程
- 生物化学 生物化学
- 分析化学 分析化学
背景情况:
- 对疾病进展的生物标志物检测在生物医学中至关重要.
- 唾液提供了一种安全,具有成本效益和非侵入性的诊断样品.
- 唾液中介质素-8 (IL-8) 水平与各种疾病有关,包括癌症和肺部疾病.
研究的目的:
- 开发一种基于光纤的新型生物传感器,用于无标签检测唾液IL-8.
- 建立一种灵敏,快速和具有成本效益的IL-8定量方法.
- 通过人工唾液样本来证明生物传感器在临床诊断方面的潜力.
主要方法:
- 开发用于蛋白质检测的光纤生物传感器.
- 唾液IL-8的无标签检测机制.
- 使用人工唾液和对照蛋白质进行验证.
主要成果:
- 实现了对IL-8的0.91 fM的超低检测极限 (LOD).
- 证明了从273 aM到59 fM的广泛测试度范围.
- 从人工唾液中的控制蛋白中显示出高灵敏度和最小的信号干扰.
结论:
- 开发的光纤生物传感器能够高灵敏,无标签地检测唾液IL-8.
- 这项技术为疾病生物标志物监测提供了一个有希望的,非侵入性的工具.
- 生物传感器的性能表明其在临床诊断和早期疾病检测中具有应用潜力.
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