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相关概念视频

Imaging Biological Samples with Optical Microscopy01:18

Imaging Biological Samples with Optical Microscopy

Optical microscopy uses optic principles to provide detailed images of samples. Antonie van Leeuwenhoek designed the first compound optical microscope in the 17th century to visualize blood cells, bacteria, and yeast cells. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes with enhanced magnification and resolution.
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...
Phase Contrast and Differential Interference Contrast Microscopy01:26

Phase Contrast and Differential Interference Contrast Microscopy

Phase-Contrast Microscopes
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...

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相关实验视频

Updated: Jul 2, 2026

Quantitative Optical Microscopy: Measurement of Cellular Biophysical Features with a Standard Optical Microscope
14:09

Quantitative Optical Microscopy: Measurement of Cellular Biophysical Features with a Standard Optical Microscope

Published on: April 7, 2014

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复杂的光学计算相位显微镜用于无标签的亚细胞成像.

Xuan Liu1, Yuwei Liu1, Shupei Yu2

  • 1Department of Electrical and Computer Engineering, New Jersey Institute of Technology, University Heights, Newark, NJ, 07102, USA.

Optics continuum
|January 15, 2024
PubMed
概括
此摘要是机器生成的。

复杂光学计算相位显微镜 (复杂-OCPM) 提供深度分辨率相位成像. 这种先进的技术实现了亚细胞分辨率,用于无标签的活细胞分析.

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Multimodal Optical Imaging Platform for Studying Cellular Metabolism
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科学领域:

  • 生物光子学 生物光子学
  • 光学成像技术的成像
  • 显微镜的使用方法

背景情况:

  • 定量相位成像为生物样本提供无标签的对比度.
  • 现有的方法在空间分辨率或深度透方面可能存在局限性.

研究的目的:

  • 调查复杂光学计算相位显微镜 (复杂-OCPM) 的性能.
  • 为了证明高空间分辨率的深度分辨率定量相位测量.

主要方法:

  • 使用低相干干扰仪与创新的光学计算方法相结合.
  • 从样本中直接测量了光学场的复杂振幅.
  • 提取的相位信息作为复杂信号的论证.

主要成果:

  • 实现了深度分辨率定量相位测量.
  • 在相位成像中展示了高空间分辨率.
  • 使用分辨率目标和活细胞验证的性能,显示亚细胞分辨率.

结论:

  • 复杂OCPM可实现高分辨率,无标签的生物样品成像.
  • 该技术提供量化阶段信息,具有深度分辨率.
  • 在没有外源标签的活细胞上实现了亚细胞分辨率.