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宫癌中miR-152的表达及其对西斯普拉丁耐药性的影响

Jun Wu^1,2, Xiaoping Chi^2,3, Qian Yang^1,2

¹Department of Obstetrics & Gynecology, The First People's Hospital of Wenling, Wenling 317500, Zhejiang Province, China.

African health sciences

|January 15, 2024

概括

微RNA 152 (miR-152) 可以在宫癌中克服西斯普拉丁耐药性. 增加miR-152表达增强了细胞亡,并减少了细胞存活,迁移和入侵,提供了一个潜在的治疗策略.

科学领域:

在瘤学瘤学.
分子生物学分子生物学
生物化学生化学

背景情况:

宫癌是一个重大的全球健康挑战,死亡率高.
了解治疗耐药性背后的分子机制对于改善患者的治疗结果至关重要.

研究的目的:

研究微RNA152 (miR-152) 在宫癌中的作用.
确定miR-152对宫癌细胞中西斯丁 (DDP) 耐药性的影响.

主要方法:

宫癌的Hela细胞被利用并分为控制,DDP,DDP +模仿nc和DDP + miR-152模仿组.
评估了细胞存活率,克隆形成,膜透率,细胞亡率和miR-152的表达.
分析了下游目标的蛋白质表达,包括ERBB3,Snail,Akt2,p-Akt和c-myc.

主要成果:

在DDP治疗组中,miR-152的表达显著上调,特别是在miR-152模仿组中.
与对照组和DDP+模仿nc组相比,miR-152模仿组表现出细胞存活率降低,克隆形成和入侵减少,亡增加.
miR-152模仿转染导致ERBB3,Snail,Akt2,p-Akt和c-myc的蛋白质表达减少.

结论:

miR-152在宫癌中起到瘤抑制作用,抑制其增殖,迁移和入侵.
miR-152通过降低ERBB3/Akt/c-myc和ERBB3/Akt/Snail通路的调节来增强对思普拉丁化疗的敏感性.

关键词:

宫癌:宫癌是一种癌症.西斯普拉丁 (cisplatin) 是一种制剂.药物耐药性耐药性药物耐药性在 miR-152 的情况下,

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