对雌激素受体α二分化和对的转录活性进行比较研究
Jong-Yeon Kim1, Yooheon Park2, Seok-Hee Lee2
1Department of Food Science and Biotechnology, Gachon University, Seongnam, 13120 Republic of Korea.
Toxicological research
|January 15, 2024
概括
大多数类都表现出雌激素活性,可能造成健康风险. 这项研究使用基于细胞的测试证实了雌激素受体α (ERα) 分解和转录激活在像乙烯和类等类中.
科学领域:
- 内分泌学和毒理学.
- 分子生物学和细胞检测
背景情况:
- 烯是消费品中广泛使用的防腐剂,包括化品和个人护理用品.
- 人们对 parabens 的潜在内分泌干扰作用,特别是其雌激素活性存在担忧.
- 帕拉本的雌激素活性可能会导致由于与雌激素受体的相互作用而导致不良健康结果.
研究的目的:
- 评估和比较各种类甲的雌激素活性,使用两种不同的基于细胞的体外测试.
- 研究 parabens 诱导雌激素受体α (ERα) 分解和转录激活的能力.
主要方法:
- 利用基于生物发光共振能量转移 (BRET) 的测定方法,使用稳定表达ERα的HEK293细胞与NanoLuc luciferase和HaloTag融合.
- 使用稳定转移的转录激活 (STTA) 试验使用ERα-HeLa9903细胞.
- 甲基 (MP),乙基 (EP),基 (PP),丁基 (BP),异基 (IsoPP) 和异基 (IsoBP) 的量化雌激素活性.
主要成果:
- 基于BRET的ERα二分化和STTA测定都显示了EP,PP,BP,IsoPP和IsoBP的雌激素活性.
- 在BRET试验中,MP显示了雌激素活性,但在STTA试验中没有.
- 测试的类甲 (EP,PP,BP,IsoPP,IsoBP) 诱导了ERα二分化和转录激活,证实了它们的雌激素潜力.
结论:
- 大多数被评估的类甲,包括EP,PP,BP,IsoPP和IsoBP,具有显著的雌激素活性.
- 这些发现突出了这些类甲与雌激素受体α通路相互作用的能力.
- 这项研究提供了体外证据,支持人们对常用类的内分泌干扰潜力的担忧.
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