Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

2.1K
Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
2.1K
Studying the Cytoskeleton01:17

Studying the Cytoskeleton

6.2K
The cytoskeletal architecture can be studied using different microscopic and biochemical techniques. Electron microscopy was instrumental in discovering the cytoskeletal architecture around the 1960s, which allowed obtaining structural information at a high-resolution level. However, the sample preparation procedure often limits this ability in biological samples. Several protocols have been developed over the years to optimize sample preparation. In one of the protocols known as rotary...
6.2K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Amidase-Catalyzed Desorption of CO<sub>2</sub> Captured in Aqueous Monoethanolamine (MEA) Solutions.

Angewandte Chemie (International ed. in English)·2026
Same author

Micro-comb 3D printing: rapid fabrication of tissue-guiding substrates using micro-embossed nozzles.

Biofabrication·2026
Same author

Liposome purification from micromolar protein background using diffusiophoretic trapping.

Nanoscale·2026
Same author

In-Solution Characterization of Biomolecular Interaction Kinetics under Native Conditions.

Analytical chemistry·2025
Same author

Standardization guidelines and future trends for PET hydrolase research.

Nature communications·2025
Same author

Comparative Study of Three Diverse Glycogen Branching Enzymes for Efficient Generation of Highly Surface Branched Starch Granules with Enhanced Digestive Resistance.

Journal of agricultural and food chemistry·2025

相关实验视频

Updated: Jul 5, 2025

Examining the Conformational Dynamics of Membrane Proteins in situ with Site-directed Fluorescence Labeling
11:55

Examining the Conformational Dynamics of Membrane Proteins in situ with Site-directed Fluorescence Labeling

Published on: May 29, 2011

15.1K

通过光显微镜揭示PET酶表面动力学.

A P Rennison1, A Nousi2, P Westh1

  • 1Department of Biotechnology and Biomedicine, Technical University of Denmark, 2800, Kongens Lyngby, Denmark.

Chembiochem : a European journal of chemical biology
|January 15, 2024
PubMed
概括

研究人员使用先进的显微镜研究了聚乙烯二甲 (PET) 水溶酶如何与PET表面结合. 这些酶表现出缓慢的溶解率,这表明表面结合动态是PET生物处理的关键.

关键词:
聚乙烯 (PET) 氧化.酶催化酶的催化作用光显微镜的光显微镜.绿色化学是一种绿色化学.接口催化剂的作用.表面动力学 表面动力学

更多相关视频

Characterizing Single-Molecule Conformational Changes Under Shear Flow with Fluorescence Microscopy
08:47

Characterizing Single-Molecule Conformational Changes Under Shear Flow with Fluorescence Microscopy

Published on: January 25, 2020

5.8K
Automated System for Single Molecule Fluorescence Measurements of Surface-immobilized Biomolecules
10:57

Automated System for Single Molecule Fluorescence Measurements of Surface-immobilized Biomolecules

Published on: November 2, 2009

12.9K

相关实验视频

Last Updated: Jul 5, 2025

Examining the Conformational Dynamics of Membrane Proteins in situ with Site-directed Fluorescence Labeling
11:55

Examining the Conformational Dynamics of Membrane Proteins in situ with Site-directed Fluorescence Labeling

Published on: May 29, 2011

15.1K
Characterizing Single-Molecule Conformational Changes Under Shear Flow with Fluorescence Microscopy
08:47

Characterizing Single-Molecule Conformational Changes Under Shear Flow with Fluorescence Microscopy

Published on: January 25, 2020

5.8K
Automated System for Single Molecule Fluorescence Measurements of Surface-immobilized Biomolecules
10:57

Automated System for Single Molecule Fluorescence Measurements of Surface-immobilized Biomolecules

Published on: November 2, 2009

12.9K

科学领域:

  • 生物化学 生物化学
  • 材料科学 材料科学 材料科学
  • 酶学 是一种酶学.

背景情况:

  • 聚乙烯二甲酸盐 (PET) 水解酶对于PET生物加工至关重要.
  • 以前的研究集中在活性部位的酶基质结合,忽视了表面相互作用.
  • 了解酶表面动态对于优化PET降解至关重要.

研究的目的:

  • 为了研究PET水解酶在PET表面上的吸附和脱附动力学.
  • 测量PHL7和LCC酶在PET上的结合动力学.
  • 开发一种酶-表面相互作用的动态模型.

主要方法:

  • 开发全内部反射光 (TIRF) 显微镜技术.
  • 在光漂白 (FRAP) 显微镜后光恢复的应用.
  • 在PET表面上测量PET水溶酶的吸附率 (启动) 和脱吸率 (关闭).

主要成果:

  • 聚乙烯水解酶 (PHL7和LCC) 显示出显著的减速率 (约为10-3s-1).
  • 这些脱离率与在其他酶系统 (如纤维素酶) 中观察到的非生产性结合相似.
  • 一个动态模型表明,吸附-脱吸动力学在PET表面的横向扩散上占主导地位.

结论:

  • 这项研究揭示了PET水解酶在PET表面的缓慢脱离动力学.
  • 酶表面动态,特别是缓慢的关闭率,对于PET生物处理至关重要.
  • 这些发现可以指导PET水解酶的工程,以增强基质向和相互作用调制.