快速启动的硫驱动的自性脱颗粒过程:细胞外电子转移途径和微生物社区进化
Wen-Jie Ma1, Han-Min Zhang1, Yu Tian2
1Key Laboratory of Industrial Ecology and Environmental Engineering (Ministry of Education, MOE), School of Environmental Science and Technology, Dalian University of Technology, No. 2 Linggong Road, Dalian 116024, PR China.
Bioresource technology
|January 15, 2024
概括
这项研究成功地缩短了硫驱动自脱 (SAD) 的启动时间,通过使用硫酸盐在颗粒污泥中丰富硫氧化细菌 (SOB). 该过程有效地切换到元素硫,改善了细胞外电子转移和颗粒结构.
科学领域:
- 环境微生物学环境微生物学
- 废水处理技术 废水处理技术
- 生物地质化学循环 生物地质化学循环
背景情况:
- 硫驱动的自脱 (SAD) 对低碳废水有效,但受到硫氧化细菌 (SOB) 缓慢生长的影响,导致启动时间长.
- 优化SOB缩对于加速SAD过程和提高效率至关重要.
研究的目的:
- 研究一种在颗粒性污泥中快速在现场丰富SOB的策略.
- 评估将电子捐赠体从硫酸硫酸盐切换为元素硫在自性脱过程中的可行性.
- 分析这种策略对颗粒结构,细胞外电子转移 (EET) 和微生物社区组成的影响.
主要方法:
- 使用无氧颗粒污泥注射启动硫酸盐驱动的自无化 (TAD).
- 在高负荷率 (176.2 g N m−3 d−1) 下,电子供体从硫酸逐渐转变为元素硫.
- 分析颗粒结构 (蛋白质二次结构),细胞外电子转移通路和微生物群落组成 (主导属).
主要成果:
- 在7天内成功启动TAD,并在第32天过渡到元素硫.
- 稳定,紧的颗粒结构保持在特定的蛋白质二次结构比率.
- 在切换到元素硫时,EET路径从间接转变为直接,而硫酸盐增强了EET酶活性.
- 主导细菌的鉴定:Thiobacillus和Sulfurimonas在TAD中,以及Longilinea在元素硫驱动的自脱过程中得到丰富.
结论:
- 拟议的战略有效地在现场丰富了SOB,大大缩短了硫驱动自脱的启动时间.
- 成功切换到元素硫证明了废水处理的成本效益高的方法.
- 了解EET机制和微生物动态为优化颗粒型污泥过程提供了洞察力.
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