相关实验视频
Updated: Aug 11, 2026

07:54
A Method to Assess Bacteriocin Effects on the Gut Microbiota of Mice
Published on: July 25, 2017
14.3K
乳酸细菌分离出传统和创新的继承者作为潜在的生物控制剂
Inês Azevedo1, Joana Barbosa1, Helena Albano2
1Universidade Católica Portuguesa, CBQF - Centro de Biotecnologia e Química Fina - Laboratório Associado, Escola Superior de Biotecnologia, Porto, Portugal.
Food microbiology
|January 15, 2024
概括
从alheiras中分离出的六种乳酸细菌菌株表现出抗菌活性,其中Lactiplantibacillus plantarum 9A3产生稳定的细菌素,对Listeria monocytogenes和Clostridium perfringens等食源病原体有效.
科学领域:
- 食品微生物学 食品微生物学
- 细菌学 细菌学是一门学科.
- 食品安全 食品安全
背景情况:
- 乳酸细菌 (LAB) 在发酵食品中至关重要,并具有抗菌特性.
- 葡萄牙传统香肠Alheiras是有益微生物的潜在来源.
- 食物传播病原体对公共健康构成重大风险,需要有效的控制策略.
研究的目的:
- 将LAB从具有抗微生物活性对食源性病原体的alheiras中分离和鉴定特征.
- 确定生产细菌素的LAB菌株,并评估它们的安全性和稳定性.
- 评估 LAB 株在食品工业中对病原体控制的潜在应用.
主要方法:
- 从传统和创新的继承者中分离和选491个LAB菌株.
- 对Listeria monocytogenes,Enterococcus faecalis,Clostridium sporogenes和Clostridium perfringens进行抗微生物活性测试. 这是一个非常好的方法.
- 全基因组测序 (WGS) 用于菌株识别和病毒毒性/耐药性基因的检测.
- 对安全方面的表型特征 (生物原胺,酶,血液溶解).
- 在各种物理和化学条件下测试细菌素的稳定性.
- 确定细菌的活性和作用方式.
主要成果:
- 六个LAB菌株表现出抗菌活性,被确定为乳球植物菌种 (2),Leuconostoc mesenteroides (1) 和Pediococcus acidilactici (3).
- 实验室发现了II类细菌素 (如Plantaricin E/F,Pediocin PA) 的基因,没有检测到毒性或抗生素耐药性基因.
- 乳植物菌植物9A3对所有测试的抗生素都表现出敏感性,并产生了对L. monocytogenes和C. perfringens有效的稳定细菌素.
- 来自Lpb的细菌素. 足部9A3在广泛的温度 (4-100°C),pH (2-8) 和化学剂中表现出稳定性,最大活性在12,800AU/mL.
- 细菌素表现出一种细菌静态的作用模式.
结论:
- 乳植物菌植物9A3是食品工业应用的安全和有前途的候选者,因为它稳定地产生细菌素.
- 鉴定到的细菌素有效地抑制了关键的食源性病原体,为食品保存提供了一种自然的方法.
- 这项研究强调了传统发酵食品中的LAB作为功能成分来源的潜力,以提高食品安全.
相关概念视频
Biological Methods for Microbial Control
Biological agents offer an effective means of controlling microbial growth by leveraging natural processes like predation, competition, and the secretion of antimicrobial substances.Predatory bacteria such as Bdellovibrio species target and kill pathogens like Salmonella and E. coli. They are widely used in poultry farms to control infections. Myxococcus species help combat plant-pathogenic fungi. These naturally occurring predators serve as eco-friendly alternatives to chemical pesticides and...
Microbes in Food Production
Microbial fermentation is central to food biotechnology, enhancing flavor, texture, preservation, and stability. Fermentative microorganisms metabolize carbohydrates into organic acids, alcohols, and other metabolites that inhibit spoilage organisms and improve digestibility while contributing distinctive sensory qualities.In baking, amylases naturally present in flour hydrolyze starch into monosaccharides such as glucose, which Saccharomyces cerevisiae ferments anaerobically. Through...
Microbes in Beverage Production
Alcoholic beverages such as wine, beer, and spirits are the products of microbial fermentation processes that transform simple sugars into ethanol and a wide array of complex flavor compounds. These transformations rely on the metabolic activities of specific yeasts and bacteria, which are selected and controlled to yield the desired beverage characteristics.Wine Fermentation and MaturationWine production begins with the crushing of grapes to release juice and pulp, forming a must that is...
Microbes in the Production of Fermented Foods
Lactic acid bacteria (LAB) and molds are instrumental in fermenting plant-based foods to enhance preservation and ensure year-round availability. These microbial processes convert plant carbohydrates into organic acids and other metabolites that inhibit spoilage organisms and contribute to the sensory qualities of the final product.In sauerkraut production, cabbage goes through a microbial succession that starts with cocci such as Leuconostoc mesenteroides. These microbes begin fermentation by...
Probiotics
Probiotics are live, non-pathogenic microorganisms that confer health benefits by modulating the gut microbiota. The human gastrointestinal tract harbors a complex microbial ecosystem, and the balance of this microbiota is crucial for digestive and systemic health. Among the most extensively studied and utilized probiotics are species formerly classified within the genera Lactobacillus and Bifidobacterium. These organisms not only naturally colonize the human gut but are also consumed through...
Production of Organic Acids
Lactic acid, an important organic acid extensively applied in food, pharmaceutical, and biodegradable polymer industries, is primarily produced via microbial fermentation. This method is favored over chemical synthesis due to its environmental sustainability and capacity for enantiomerically pure product formation. Among various microbial processes, the fermentation of starch-based substrates stands out due to the abundance and renewability of raw materials like corn and potatoes.Hydrolysis of...

