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在Helicoverpa armigera中,V-ATPase E调解了Cry2Ab结合和毒性

Yuge Zhao¹, Pin Li², Xue Yao²

¹College of Life Sciences, Henan Agricultural University, Zhengzhou 450046, China; State key Laboratory of Wheat and Maize Crop Science/College of Plant Protection, Henan Agricultural University, Zhengzhou 450046, China.

Pesticide biochemistry and physiology

|January 15, 2024

概括

真空H+-ATPase E亚单元 (V-ATPase E) 作为Helicoverpa armigera.中的Cry2Ab杀虫蛋白的受体. 这种相互作用增强了Cry2Ab的毒性,提供了一种管理害虫耐药性的策略.

科学领域:

农业昆虫学农业昆虫学
分子生物学分子生物学
生物化学生物化学

背景情况:

细菌 thuringiensis (Bt) Cry2Ab 蛋白质提供了一个替代方法来控制对 Cry1A 毒素有耐药性的昆虫害虫.
空腔H+-ATPase亚单元A和B (V-ATPase A和B) 已与Bt蛋白的杀虫活性有关.

研究的目的:

调查V-ATPases亚单元E在Helicoverpa armigera的Cry2Ab毒性中的作用.
为了确定V-ATPases E作为Cry2Ab.的潜在受体.

主要方法:

用RT-PCR分析暴露于Cry2Ab.的H. armigera幼虫中的基因表达变化.
联结斑,同类和异质竞争试验验证蛋白质结合.
在Sf9细胞中异质表达和在H. zea中肠细胞中基因敲除以评估功能作用.
在体内生物测试使用H. armigera幼虫.

主要成果:

暴露于Cry2Ab显著降低了H. armigera V-ATPase E (HaV-ATPase E) 的表达.
哈维-ATPase E 物理和特定地与激活的 Cry2Ab 毒素结合.
在表达 HaV-ATPase E. 的 Sf9 细胞中增强 Cry2Ab 敏感性.
在减少V-ATPase E.的H. zea细胞中降低了Cry2Ab细胞毒性.
增加了H. armigera幼虫死亡率,当Cry2Ab与HaV-ATPase E蛋白一起食时.

结论:

V-ATPases E 是 H. armigera. 中 Cry2Ab 的一个假定受体.
V-ATPases E可以增强Cry2Ab的毒性.
准V-ATPases E可能为管理昆虫害虫中的Cry2Ab耐药性提供了一种策略.

关键词:

有约束力的约束力在Cry2AbAb中,我们可以看到Cry2Ab.这是一架直升机的朋友.接收器接收器接收器有毒性有毒性在V-ATPase E中使用.

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