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一种编码蛋白质的CCDC7循环RNA通过上调FLRT3来抑制前列腺癌的进展

Qiong Wang^1,2, Bisheng Cheng³, Sandeep Singh²

¹Department of Urology, Nanfang Hospital, Southern Medical University, Guangzhou, 510515, China.

NPJ precision oncology

|January 15, 2024

概括

一种新型的蛋白质编码圆形RNA,circCCDC7,在前列腺癌中起到瘤抑制作用. 它编码的蛋白质抑制了癌细胞的迁移和入侵,提供了潜在的治疗应用.

科学领域:

分子生物学分子生物学
在瘤学瘤学.
遗传学是一个遗传学.

背景情况:

循环RNAs (circRNAs) 越来越多地被认为具有不同的生物作用,包括基因调节.
环RNAs的蛋白质编码潜力在很大程度上仍未被探索,这代表了新型蛋白质的潜在来源.
前列腺癌的进展与复杂的分子变化有关,需要确定新的治疗点.

研究的目的:

在前列腺癌中识别和描述新的蛋白质编码circRNAs.
在前列腺癌中研究circCCDC7的功能作用.
探索circCCDC7 ((15,16,17,18,19) 和其编码蛋白的治疗潜力.

主要方法:

生物信息分析以确定候选circRNAs.
分子克隆和证实circCCDC7的有效性.
在体外测试以评估细胞迁移,入侵和活力.
西方斑点和有条件介质实验以确认蛋白质分泌和功能.

主要成果:

circCCDC7 ((15,16,17,18,19) 被确定为一种来自CCDC7基因的编码蛋白 circRNA.
circCCDC7 ((15,16,17,18,19) 表达在高格里森评分前列腺癌中下调,与预后不佳相关.
过度表达circCCDC7 ((15,16,17,18,19) 抑制前列腺癌细胞迁移,入侵和活力,作为瘤抑制剂.
瘤抑制活性由分泌的蛋白质 (circCCDC7-180aa) 介导,这也影响了FLRT3的表达.

结论:

circCCDC7 ((15,16,17,18,19) 是前列腺癌中的瘤抑制剂,通过其编码的分泌蛋白发挥作用.
circCCDC7-180aa代表了一种用于前列腺癌治疗的新型治疗性.
circCCDC7 ((15,16,17,18,19) 和其蛋白质产品对未来的前列腺癌疗法充满希望.

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