基因激活蛋白激酶模块CcSte11-CcSte7-CcPmk1通过Cytospora chrysosperma中的转录因子CcSte12调节病原性
Lu Yu1,2, Yuchen Yang1,2, Xiaolin Qiu1,2
1State Key Laboratory of Efficient Production of Forest Resources, Beijing, China.
Stress biology
|January 15, 2024
概括
这项研究揭示了Cytospora chrysosperma中的CcPmk1-MAPK信号通路对于树癌症至关重要. 关键组件CcSte11和CcSte7调节下游目标,如CcSte12,影响真菌毒性.
科学领域:
- 植物病理学 植物病理学
- 分子菌学 分子菌学.
- 信号转导 信号转导
背景情况:
- 植物 (Cytospora chrysosperma) 由于树的癌症,造成了巨大的经济损失.
- 线素激活蛋白激酶 (MAPK) 级联对于真菌病原性至关重要,Pmk1-MAPK在植物病原体中至关重要.
- 之前的研究已经确定了CcPmk1作为C. chrysosperma中致病性的关键调节者.
研究的目的:
- 为了识别和描述CCPmk1 MAPK级联的上游组件.
- 阐明CcPmk1下游的监管网络,重点关注CcSte12.
- 了解CcPmk1-MAPK途径在C. chrysosperma病原性中的作用.
主要方法:
- 基因删除和CcSte11和CcSte7突变的特征.
- 在MAPK级联 (CcSte11,CcSte7,CcPmk1,CcSte50) 中对蛋白质与蛋白质相互作用的分析.
- 在模拟感染期间,与野生类型相比,对CcSte12删除突变的转录分析,包括GO和KEGG通路丰富分析.
主要成果:
- 删除CcSte11和CcSte7导致生长受损,化和毒性,反映了CcPmk1删除表型.
- 已证明CcSte11,CcSte7,CcPmk1和CcSte50相互作用,形成一个信号复合体.
- 转录分析显示,CcSte12调节酶活性和粉/糖代谢,在删除突变中降低了甘氨基酶和效应基因的调节. 在CcPmk1和CcSte12调节之间发现了116个重叠基因.
结论:
- 涉及CcSte11,CcSte7,CcPmk1和CcSte50的CcPmk1-MAPK通路作为一个关键信号传导模块.
- 通过CcSte12的下游调节控制了重要的毒性因子,包括水解酶和效应因子.
- 这种CcPmk1-MAPK通路对于Cytospora chrysosperma的致病性是不可或缺的.
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