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毛囊式电动力学染色学用于奇拉分离潜在的SARS-CoV-2 3CL蛋白酶抑制剂

Lucile Brier¹, Christophe Furman², Julie Charton¹

¹University of Lille, Inserm, Institut Pasteur de Lille, U1177 - Drugs and Molecules for Living Systems, Lille, France.

Electrophoresis

|January 16, 2024

概括

一种新的毛细管电泳法有效地确定了潜在的SARS-CoV-2 3CL蛋白酶抑制剂的酶体纯度. 这种分析工具提供了出色的分离性能和环保性,优于经过测试的HPLC方法.

科学领域:

分析化学分析化学
药品分析药品分析
绿色化学绿色化学

背景情况:

奇拉尔化合物在药物开发中至关重要,需要强大的方法来评估体纯度.
严重急性呼吸系统综合征冠状病毒2 (SARS-CoV-2) 3CL蛋白酶抑制剂是重要的治疗标.
对于药物的有效性和安全性来说,对体纯度的质量控制至关重要.

研究的目的:

开发和验证一种毛细电泳 (CE) 方法,用于确定五种性化合物的性纯度.
评估开发的CE方法的分析和环境性能.
为了将CE方法的分离能力与目标化合物的高性能液态染色学 (HPLC) 进行比较.

主要方法:

毛细管电泳 (CE) 使用高硫化β-环氧化的性选择器.
用聚乙烯氧化物进行动态毛细血管涂层.
优化缓冲组合,电压和温度,以实现对抗分子分离.
高性能液态染色学 (HPLC) 具有两种不同的奇拉静止相.

主要成果:

开发的CE方法成功地分离了所有五种奇拉衍生物.
该方法表现出良好的分析性能,具有计算的检测和量化极限.
该CE方法获得了有利的绿色评分,表明了环境可持续性.
使用经过测试的奇拉静止相的HPLC方法未能完全分离所有衍生物.

结论:

使用高硫酸β-环氧的毛细管电泳是一种适合和绿色的质量控制工具,用于评估潜在的SARS-CoV-2 3CL蛋白酶抑制剂的酶体纯度.
与这些特定化合物的评估HPLC条件相比,CE方法提供了更高的分离效率.
这种CE方法为制药开发和质量保证提供了有价值的分析方法.

关键词:

毛细血管电泳毛细血管电泳性分离是指性分离.绿色指标绿色指标高性能液体色谱学高性能液体色谱学

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