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全基因组关联分析确定了一个候选基因控制种子大小和产量在Xanthoceras sorbifolium Bunge中

Ziquan Zhao¹, Chongjun Liang^1,2, Wei Zhang¹

¹State Key Laboratory of Tree Genetics and Breeding, Research Institute of Forestry Chinese Academy of Forestry, Beijing 100091, China.

Horticulture research

|January 16, 2024

概括

研究人员在黄色角中发现了一种基因XsAP2,该基因可能会负面调节种子产量. 这一发现为培育产量更高的黄角 (Xanthoceras sorbifolium) 品种提供了洞察力.

科学领域:

植物遗传学植物遗传学
分子生物学分子生物学
农业科学农业科学

背景情况:

黄角 (Xanthoceras sorbifolium) 是一种有价值的油树,但其种子产量的遗传基础尚不清楚.
黄角种子油富含有益的脂肪酸,适用于食用油和生物柴油.
了解控制种子产量的遗传因素对于改善这一重要的作物至关重要.

研究的目的:

为了确定与黄角种子产量特征相关的遗传位置.
调查候选基因影响种子产量的功能.
为提供高产黄角分子育种的基础.

主要方法:

全基因组再测序和全基因组关联研究 (GWAS) 在222个黄色角生殖质上.
使用桑格测序识别和验证重要的SNP位点.
基因表达分析 (RT-qPCR) 和功能分析在转基因Arabidopsis.

主要成果:

确定了399个与百粒重量,单果种子质量和种子数量相关的显著SNP位点.
发现了一个候选基因,XsAP2 (AP2转录因子),位于重要的位置附近.
XsAP2在种子中表达高,与种子重量和质量负相关;过度表达减少了Arabidopsis.种子的种子产量.

结论:

基因XsAP2可能在黄角种子产量方面发挥负面调节作用.
这项研究提供了宝贵的遗传资源和候选基因,用于黄色角种植.
这些发现有助于分子理解和改善黄角种子生产.

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