肠道微生物发酵产品Pleurotus eryngii多糖与肠道粘液之间的相互作用
Gaoxing Ma1, Sai Ma1, Hengjun Du2
1Collaborative Innovation Center for Modern Grain Circulation and Safety, Jiangsu Province Engineering Research Center of Edible Fungus Preservation and Intensive Processing, College of Food Science and Engineering, Nanjing University of Finance and Economics, Nanjing 210023, China.
Food & function
|January 16, 2024
概括
发酵的Pleurotus eryngii多糖 (FPEP) 与肠道粘液相互作用,减少聚合. 发酵时间影响这种相互作用,影响FPEPEP.
科学领域:
- 胃肠道学和肠道微生物群研究
- 食品科学和多糖化物化学 食品科学和多糖化物化学
背景情况:
- 红素多糖 (PEP) 是一种关键的生物活性化合物,影响肠道微生物群.
- PEP与肠粘液层 (IML) 和上皮细胞的相互作用对其生物效应至关重要.
研究的目的:
- 描述微生物降解后发酵P. eryngii多糖 (FPEP) 和肠粘液 (IM) 之间的相互作用.
- 调查发酵时间如何影响FPEP-IM相互作用和多糖聚合.
主要方法:
- 扫描电子显微镜 (SEM) 用于可视化FPEP-IM相互作用.
- 颗粒大小分析以量化IM对FPEP聚合的影响.
- 度测量 (OD值) 以评估FPEP-IM随时间的相互作用动态.
主要成果:
- FPEP与IM相互作用,导致减少多糖分子聚合.
- 在IM的存在下,SEM揭示了FPEP的分散,与溶液中的聚合形成鲜明对比.
- 在0h发酵时,IM (485.1nm) 与没有IM (989.33nm) 的颗粒大小显着较小.
- 添加IM的最大颗粒大小发生在24小时发酵 (585.87 nm) 后.
- 度测试显示,在12小时的相互作用期间,24小时发酵FPEP的OD值始终高.
结论:
- 发酵P. eryngii多糖 (FPEP) 与肠粘液 (IM) 相互作用,调节其物理特性.
- 相互作用减少FPEP聚合,并影响其颗粒大小,发酵时间是关键因素.
- 这项研究为了解PEP吸收和运输提供了基础,为肠道消化提供了新的见解.
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