一个保存的转录因子控制葡萄糖生成通过不同的目标在高盐水适应的古生物与多样化的代谢能力
Rylee K Hackley1,2, Angie Vreugdenhil-Hayslette1, Cynthia L Darnell1
1Department of Biology, Duke University, Durham, North Carolina, United States of America.
PLoS genetics
|January 16, 2024
概括
在Haloarcula hispanica中的TrmB调节器控制葡萄糖生成和酸盐生物合成,与其在Halobacterium salinarum中的作用不同. 它的功能随着古生物的代谢网络变化而演变.
科学领域:
- 微生物学 微生物学
- 分子生物学分子生物学
- 生物化学 生物化学
背景情况:
- 细胞过程需要及时调节碳代谢,以防止徒劳的循环.
- 在Halobacterium salinarum中的TrmB家族蛋白调节葡萄糖生成和生物合成,尽管archaeon不利用碳水化合物作为能量.
研究的目的:
- 调查TrmB目标和功能在西班牙甲状腺 (Haloarcula hispanica) 中的保存情况,这是一个糖溶性古物质.
- 了解TrmB调节在具有不同代谢能力的古生物中如何不同.
主要方法:
- 在Haloarcula hispanica中对TrmB家族转录调节器的表征.
- 对TrmB结合部位和基因表达特征的全基因组分析.
主要成果:
- 在哈尔的TrmB. 西班牙人结合到15个基因组位点,诱导葡萄糖生成和酸盐生物合成的基因.
- 在Hbt.观察到的关键监管控制. 盐酸 (ppsA激活,pykA抑制) 在哈尔没有. 西班牙裔. 西班牙裔.
- 在Har.中,TrmB并没有全球调节糖解酶或其他碳代谢途径. 西班牙裔. 西班牙裔.
结论:
- 在Haloarchaea中的TrmB regulon被重新连接,反映了代谢网络的演变.
- 在具有不同代谢策略的古物种中,TrmB的调节作用并未在全球范围内得到保护.
相关概念视频
Prokaryotic Transcriptional Activators and Repressors
21.1K
The organization of prokaryotic genes in their genome is notably different from that of eukaryotes. Prokaryotic genes are organized, such that the genes for proteins involved in the same biochemical process or function are located together in groups. This group of genes, along with their regulatory elements, are collectively known as an operon. The functional genes in an operon are transcribed together to give a single strand of mRNA known as polycistronic mRNA.
Transcription of prokaryotic...
Transcription of prokaryotic...
21.1K
General Transcription Factors
5.3K
Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...
5.3K
Transcription Attenuation in Prokaryotes
15.3K
Transcriptional attenuation occurs when RNA transcription is prematurely terminated due to the formation of a terminator mRNA hairpin structure. Bacteria use these hairpins to regulate the transcription process and control the synthesis of several amino acids including histidine, lysine, threonine, and phenylalanine. Transcription attenuation takes place in the non-coding regions of mRNA.
There are several different mechanisms used to attenuate transcription. In ribosome mediated...
There are several different mechanisms used to attenuate transcription. In ribosome mediated...
15.3K
Co-activators and Co-repressors
7.4K
Gene transcription is regulated by the synergistic action of several proteins that form a complex at a gene regulatory site. This is observed in eukaryotes, where the regulation of gene expression is a complex process. Regulatory proteins in eukaryotes can broadly be classified into two types – regulators that bind directly to specific DNA sequences and co-regulators that associate with regulatory proteins but cannot directly bind to the DNA. These co-regulators are further divided into...
7.4K
Transcription Factors
75.9K
Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...
75.9K
Operons
49.1K
Prokaryotes can control gene expression through operons—DNA sequences consisting of regulatory elements and clustered, functionally related protein-coding genes. Operons use a single promoter sequence to initiate transcription of a gene cluster (i.e., a group of structural genes) into a single mRNA molecule. The terminator sequence ends transcription. An operator sequence, located between the promoter and structural genes, prohibits the operon’s transcriptional activity if bound by...
49.1K


