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Updated: Jul 5, 2025

Synthesis of 1,2-Azaborines and the Preparation of Their Protein Complexes with T4 Lysozyme Mutants
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Published on: March 25, 2017

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在l-伊索瓦林生物合成中形成阿齐里丁的α-甲酸依赖氧基酶的表征

Lu Zhou1,2, Takayoshi Awakawa1,2, Richiro Ushimaru1,3

  • 1Graduate School of Pharmaceutical Sciences, The University of Tokyo, 7-3-1, Bunkyo-ku, Tokyo 113-0033, Japan.

Organic letters
|January 16, 2024
PubMed
概括

研究人员发现了Tolypocladium膨胀酶如何产生l-isovaline. 一个特定的酶突变改变了立体选择性,影响了最终的d-异氨酸产品,为生物合成途径提供了洞察力.

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Preparation of 1° Amines: Azide Synthesis

Direct alkylation of ammonia produces polyalkylated amines, along with a quaternary ammonium salt. To exclusively prepare primary amines, the azide synthesis method can be used.
Azide ions act as good nucleophiles and react with unhindered alkyl halides to form alkyl azides. Alkyl azides do not participate in further nucleophilic substitution reactions, thereby eliminating the chances of polyalkylated products. Alkyl azides are reduced by hydride-based reducing agents, like lithium aluminum...
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Aldehydes and Ketones with Amines: Imine Formation Mechanism

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Imines are formed under mildly acidic conditions. A pH of 4.5 is ideal for the reaction.
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Allosteric Proteins-ATCase

Binding sites linkages can regulate a protein's function.  For example, enzyme activity is often regulated through a feedback mechanism where the end product of the biochemical process serves as an inhibitor.
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