相关实验视频

Updated: Jul 5, 2025

Isolation and Cultivation of Neural Progenitors Followed by Chromatin-Immunoprecipitation of Histone 3 Lysine 79 Dimethylation Mark

Isolation and Cultivation of Neural Progenitors Followed by Chromatin-Immunoprecipitation of Histone 3 Lysine 79 Dimethylation Mark

Published on: January 26, 2018

通过H3K9me2修饰,PCGF6控制着小鼠的Tuft细胞分化,独立于Polycomb抑制

Annachiara Del Vecchio¹, Patrizia Mulé¹, Daniel Fernández-Pérez¹

¹IEO, European Institute of Oncology IRCCS, Department of Experimental Oncology, Via Adamello 16, 20139 Milan, Italy.

Developmental cell

|January 16, 2024

概括

波利康布蛋白PCGF6控制小鼠肠道Tuft细胞分化独立于正规的波利康布抑制复合体1 (PRC1) 途径. 这一发现揭示了PCGF6在维持成年组织平衡中具有新的Polycomb-独立的作用.

科学领域:

细胞生物学细胞生物学
分子生物学分子生物学
发育生物学是发展生物学.

背景情况:

细胞命运的决定依赖于转录程序,这些程序对组织平衡和再生至关重要.
聚合物抑制综合体1 (PRC1),含有E3酶RING1A和B,沉积H2AK119ub1,对肠道干细胞功能至关重要.
在活体中,不同PRC1对应物 (PCGF1-6) 的特定作用在很大程度上是未知的.

研究的目的:

为了研究PCGF6在小鼠肠道恒温的功能.
确定PCGF6在细胞分化中的作用.
阐明PCGF6的功能背后的机制,特别是它与H2AK119ub1沉积的独立性.

主要方法:

在小鼠肠道中分析PCGF6染色质占用率.
调查PCGF6与调控元素和基因素修饰的关联.
评估PCGF6失活对细胞分化的影响.
将PCGF6功能丧失表型与RING1A/B损失进行比较.

主要成果:

PCGF6 调节了小鼠肠道细胞分化,独立于 H2AK119ub1.1.
结合PCGF6染色体的作用超越了Polycomb域,扩展到独特的调节元素.
PCGF6与MGA相互作用,用于E-BOX识别,并调解H3K9me2促进体沉积.
由于PCGF6的不活化导致了上细胞的自主积累,与RING1A/B损失不同.

结论:

PCGF6在细胞分化和肠道平衡中发挥着至关重要的作用.
在成年组织中,PCGF6表现出多胞体独立的功能.
对于PCGF6的监管作用,它使用了涉及MGA和H3K9me2的不同的机制.

关键词:

H2AK119ub1 在线观看 H3K9me2是什么意思 H3K9me2是什么意思在PCGF6中.在PRC1中,聚合的多元.在RING1A/B上使用.状细胞是状细胞.细胞身份是细胞的身份.在这种情况下,染色染色素转录转录是一种转录.

更多相关视频

Reprogramming Mouse Embryonic Fibroblasts with Transcription Factors to Induce a Hemogenic Program

Reprogramming Mouse Embryonic Fibroblasts with Transcription Factors to Induce a Hemogenic Program

Published on: December 16, 2016

Repressing Gene Transcription by Redirecting Cellular Machinery with Chemical Epigenetic Modifiers

Repressing Gene Transcription by Redirecting Cellular Machinery with Chemical Epigenetic Modifiers

Published on: September 20, 2018

相关概念视频

Combinatorial Gene Control

Combinatorial Gene Control

Combinatorial gene control is the synergistic action of several transcriptional factors to regulate the expression of a single gene. The absence of one or more of these factors may lead to a significant difference in the level of gene expression or repression.
The expression of more than 30,000 genes is controlled by approximately 2000-3000 transcription factors. This is possible because a single transcription factor can recognize more than one regulatory sequence. The specificity in gene...

Master Transcription Regulators

Master Transcription Regulators

Master transcription regulators are regulatory proteins that are predominantly responsible for regulating the expression of multiple genes. Often these genes work in concert to drive a complex process. Activation of a master transcription regulator can lead to a cascade of transcriptional activation necessary for that outcome. These regulators can directly bind to the regulatory sequences of the various genes involved, or they can indirectly regulate transcription by binding to regulatory...