相关实验视频

Updated: Jul 5, 2025

Formulations for Freeze-drying of Bacteria and Their Influence on Cell Survival

Formulations for Freeze-drying of Bacteria and Their Influence on Cell Survival

Published on: August 3, 2013

冷和干燥程序对微波冷干燥过程中的IgY聚合和活性的影响:三糖和曼尼托尔的保护作用和相互作用

Zhe Wang¹, Wenchao Liu², Xu Duan²

¹School of Food and Biobiological Engineering, Henan University of Science and Technology, 471000 Luoyang, China; Food Laboratory of Zhongyuan, 462300 Luohe, China.

International journal of biological macromolecules

|January 16, 2024

概括

高质量的冷化IgY (免疫球蛋白Y) 需要优化冷干燥. 微波冷干燥 (MFD) 可以导致聚合,但三醇-曼尼托混合物以 1:3 的比例有效地保持抗体活性.

科学领域:

生物化学生物化学
材料科学材料科学材料科学
免疫学免疫学免疫学

背景情况:

高质量的冷化IgY (免疫球蛋白Y) 对于需要稳定性和保存活性的应用至关重要.
目前用于IgY制备的方法需要优化,以防止聚合并保持有效性.
研究新的冷干燥技术对于提高IgY产品质量至关重要.

研究的目的:

评估微波冷干燥 (MFD) 作为制备冷化IgY的方法.
评估MFD对IgY聚合和结构完整性的影响.
为了确定最佳的冷保护剂和减轻MFD期间IgY聚合的比率.

主要方法:

对IgY经过冷和MFD的形态评估.
使用特雷和曼尼托尔等保护剂分析IgY聚合.
与酶相关的免疫吸收试验 (ELISA) 用于测量IgY结合亲和力和抗体活性.
不同的三醇与曼尼托尔比率的比较分析.

主要成果:

低温冷和MFD都诱导了IgY聚合,MFD的影响更为明显.
一种由三糖和曼尼托尔组成的复合防护剂显著降低了IgY聚合.
最佳的三醇与曼尼托尔比率 (1:3) 最小化了产品崩和IgY聚合.
这种最佳比率导致活性IgY抗体的保存率达到78.57%.

结论:

微波冷干燥 (MFD) 可能是IgY制备的可行方法,但需要冷保护剂来防止聚合.
特雷和曼尼托尔,特别是以1:3的比例,在MFD期间有效地保护IgY结构和活性.
优化的MFD协议与适当的冷保护剂可以产生高质量的冷化IgY,具有增强的稳定性和保存的抗体功能.

关键词:

与焦点相对应的激光扫描显微镜.这就是IgYY.热保护剂热保护剂在MFDD中,MFD是最重要的.蛋白质结构蛋白质结构

更多相关视频

Identification of Plant Ice-binding Proteins Through Assessment of Ice-recrystallization Inhibition and Isolation Using Ice-affinity Purification

Identification of Plant Ice-binding Proteins Through Assessment of Ice-recrystallization Inhibition and Isolation Using Ice-affinity Purification

Published on: May 5, 2017

Measuring the Densities of Aqueous Glasses at Cryogenic Temperatures

Measuring the Densities of Aqueous Glasses at Cryogenic Temperatures

Published on: June 28, 2017

相关概念视频

Responses to Heat and Cold Stress

Responses to Heat and Cold Stress

Every organism has an optimum temperature range within which healthy growth and physiological functioning can occur. At the ends of this range, there will be a minimum and maximum temperature that interrupt biological processes.

Cryo-electron Microscopy

Cryo-electron Microscopy

Conventional electron microscopy (EM) involves dehydration, fixation, and staining of biological samples, which distorts the native state of biological molecules and results in several artifacts. Also, the high-energy electron beam damages the sample and makes it difficult to obtain high-resolution images. These issues can be addressed using cryo-EM, which uses frozen samples and gentler electron beams. The technique was developed by Jacques Dubochet, Joachim Frank, and Richard Henderson, for...