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在可逆和可溶性载体上联合固定的多酶生物催化系统用于玉米草纤维素的糖化
Xiaoxiao Yu1, Jianzhen Li1, Yan Sun1
1College of Life Science, Jilin Agricultural University, Changchun 130118, China; Key Laboratory of Straw Comprehensive Utilization and Black Soil Conservation, The Ministry of Education, Jilin Agricultural University, Changchun 130118, China.
Bioresource technology
|January 16, 2024
概括
三种协同作用的酶被共同固定,以有效地降解玉米中的纤维素. 新型载体允许酶恢复和重复使用,在多个循环中保持显著的活性.
科学领域:
- 生物催化剂是一种生物催化剂.
- 生物技术是生物技术.
- 酵素工程是什么意思 酵素工程
背景情况:
- 酶固定对于工业应用至关重要.
- 多个酶的联合不动化可以增强协同效应.
- 尤德拉吉特L-100为酶恢复提供可逆和可溶性质.
研究的目的:
- 在Eudragit L-100上联合固定细胞酶,β-葡萄糖酶和pectinase.
- 通过使用联合不动化的酶来研究草纤维素的协同降解.
- 评估共固定酶系统的恢复和可重复使用性.
主要方法:
- 在Eudragit L-100.00上对细胞酶,β-葡萄糖酶和pectinase进行联合固定.
- 为纤维素水解优化酶比例和反应条件.
- 评估多个循环的酶活性恢复和保留.
主要成果:
- 从玉米草获得了74.45%的纤维素转化率.
- 在5个循环后,共同固定化的酶保持了54.67%的活性.
- 由于载体的特性,可以成功从固体基板中恢复酶.
结论:
- 在Eudragit L-100上对协同酶的联合不动化对纤维素降解是有效的.
- 可逆和可溶性载体促进了酶的恢复和重复使用,提高了过程的可持续性.
- 这种方法显示了对基纤维素生物质的高效和经济的生物转化潜力.
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