相关实验视频

Updated: Jul 5, 2025

Adoptive Transfer of IL-33-Stimulated Macrophages into Bleomycin-Induced Mouse Models to Study Their Effect on Idiopathic Pulmonary Fibrosis In Vivo

Adoptive Transfer of IL-33-Stimulated Macrophages into Bleomycin-Induced Mouse Models to Study Their Effect on Idiopathic Pulmonary Fibrosis In Vivo

Published on: May 5, 2023

血提取物通过TGF-β1/Smad3信号通路缓解异常性肺纤维化

Yin Zhang¹, Yong-Bo Lu¹, Wei-Jie Zhu²

¹Jiangsu Key Laboratory for High Technology Research of TCM Formulae, National and Local Collaborative Engineering Center of Chinese Medicinal Resources Industrialization and Formulae Innovative Medicine, Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, Nanjing University of Chinese Medicine, Nanjing 210023, P. R. China.

Journal of ethnopharmacology

|January 16, 2024

概括

超过10KDa的血清提取物成分通过降低对原蛋白和纤维蛋白的调节,显著抑制肺纤维化. 这部分调节酸盐激酶M2和Smad7,为肺纤维化提供了一种新的治疗策略.

科学领域:

药理学药理学是指药理学的学科.
传统中国医药传统中国医药
肺纤维化研究研究

背景情况:

是一种传统的中医药,用于血液循环和静止,具有治疗肺纤维化 (PF) 的历史应用.
含有血清的制剂已在异形性肺纤维化 (IPF) 中显示出临床益处,但活性成分尚不清楚.

研究的目的:

为了识别具有抗肺纤维化作用的水成分.
阐明这些活跃的水成分的治疗机制.

主要方法:

根据分子量 (>10 kDa,3-10 kDa,3 kDa) 分割的水提取物.
使用TGF-β1诱导纤维细胞 (MTT,伤口愈合,IF,WB) 的体外研究.
在体内研究使用白胺诱导的肺纤维化小鼠模型 (H&E,Masson,HYP,WB,IF).

主要成果:

超过10kDa的吸血虫提取物分量在体外抑制了纤维细胞的增殖和迁移.
这种分量减少了原和纤维内素沉积,并在体内改善了肺组织结构.
超过10kDa的分量上调了pyruvate kinase M2 (PKM2) 和Smad7的表达,这是缓解纤维化的关键.

结论:

吸血虫提取物的>10kDa分量是抑制肺纤维化的主要成分.
该机制涉及TGF-β1/Smad3信号通路,由PKM2和Smad7.7调节.

关键词:

异形性肺纤维化症异形性肺纤维化症李奇 (Leech) 是一个鱼.酸盐激酶M2的使用方法在TGF-β1/Smad3信号通路中.含有分子量大于10KDa的吸血虫提取物组.

更多相关视频

A Mouse Model of Pulmonary Fibrosis Induced by Nasal Bleomycin Nebulization

A Mouse Model of Pulmonary Fibrosis Induced by Nasal Bleomycin Nebulization

Published on: January 20, 2023

Oropharyngeal Administration of Bleomycin in the Murine Model of Pulmonary Fibrosis

Oropharyngeal Administration of Bleomycin in the Murine Model of Pulmonary Fibrosis

Published on: May 9, 2025

相关概念视频

TGF - β Signaling Pathway

TGF - β Signaling Pathway

The TGF-β signaling pathway regulates cell growth, differentiation, adhesion, motility, and development. TGF-β ligands that induce TGF-β signaling are synthesized in their latent form. Several proteases or cell surface receptors such as integrins act upon the latent form, releasing the active ligand. There are three types of mammalian TGF-βs: (TGF-β1, TGF-β2, and TGF-β3) that bind as homodimers or heterodimers to TGF-β receptors. The TGF-β receptors...