相关实验视频

Updated: Aug 5, 2026

Synthesis of Thermogelling PolyN-isopropylacrylamide-graft-chondroitin Sulfate Composites with Alginate Microparticles for Tissue Engineering

Synthesis of Thermogelling PolyN-isopropylacrylamide-graft-chondroitin Sulfate Composites with Alginate Microparticles for Tissue Engineering

Published on: October 26, 2016

开发糖/酸聚合物支用于胡卜的过氧化酶固定及其在基去除中的应用

Saleh A Mohamed¹, Alshaimaa M Elsayed², Hala A Salah²

¹Molecular Biology Department, National Research Centre, Dokki, Cairo, Egypt. saleh38@hotmail.com.

Scientific reports

|January 16, 2024

概括

和酸盐形成了一个新型的支物,用于固定胡卜过氧化酶 (HRP). 这种ACG-HRP结合物表现出增强的稳定性,可重复使用性和降解污染物的效率,对生物技术用途具有前景.

科学领域:

生物材料科学生物材料科学
酶固定化酶固定化
生物技术是生物技术.

背景情况:

酶固定对于工业应用至关重要,需要稳定高效的支持矩阵.
糖 (CG) 和酸 (A) 具有独特的结构性质,适合酶支持发育.

研究的目的:

合成一种新型的果/酸 (ACG) 聚合物复合物,以支持胡卜多氧化酶 (HRP) 固定.
描述固定酶 (ACG-HRP) 并评估其用于生物技术应用的特性.

主要方法:

合成ACG聚合物复合物和HRP的固定.
使用FTIR,SEM和TGA进行表征.
评估酶活性,稳定性,可重复使用性和基质亲和力.

主要成果:

在特定条件下达到75%的最高固定恢复率 (1.0%CG,2%A,pH7.0,50单位HRP).
与可溶性HRP相比,ACG-HRP表现出更好的热稳定性,更高的温度最佳 (50°C) 和酸性pH最佳 (6.0).
证明了良好的可重复使用性 (60%在10个循环中) 和提高降解和p-chlorofenol的效率,改善了对抑制剂的耐药性.

结论:

开发的ACG聚合物支有效地固定HRP,增强其性能和稳定性.
ACG-HRP显示出各种生物技术应用的巨大潜力,包括生物修复.
这项研究强调了结合糖和酸用于酶固定化的协同效益.

更多相关视频

Immobilization of Multi-biocatalysts in Alginate Beads for Cofactor Regeneration and Improved Reusability

Immobilization of Multi-biocatalysts in Alginate Beads for Cofactor Regeneration and Improved Reusability

Published on: April 22, 2016

3D Printing of In Vitro Hydrogel Microcarriers by Alternating Viscous-Inertial Force Jetting

3D Printing of In Vitro Hydrogel Microcarriers by Alternating Viscous-Inertial Force Jetting

Published on: April 21, 2021

相关概念视频

Microbial Fermentation

Microbial Fermentation

Fermentation is a crucial anaerobic metabolic process that enables microbes to derive energy from sugar without relying on oxygen or an electron transport chain. This process is fundamental to various biological and industrial applications and is classified based on the metabolic products generated.Role of Pyruvate in FermentationPyruvate and its derivatives serve as key electron acceptors in fermentative pathways. The oxidation of NADH to regenerate NAD+ is essential for the continuation of...

Biosynthesis of Polysaccharides

Biosynthesis of Polysaccharides

Polysaccharides such as glycogen and starch are synthesized from nucleoside diphosphate sugars, primarily uridine diphosphate glucose (UDPG) and adenosine diphosphate glucose (ADPG). These activated glucose donors act as key intermediates in carbohydrate metabolism and biosynthesis. UDPG primarily involves glycogen synthesis in animals and many bacteria, while ADPG plays a fundamental role in starch synthesis in plants and certain bacteria.UDPG is formed when glucose-1-phosphate reacts with...

Production of Alcohol

Production of Alcohol

Continuous fermentation is a key strategy in industrial ethanol production, particularly when efficiency, scalability, and high yields are essential. This approach allows for uninterrupted operation and optimized resource utilization. The primary feedstock, corn starch, undergoes enzymatic hydrolysis facilitated by α-amylase and glucoamylase. These enzymes break down the starch into fermentable sugars such as glucose, which are readily assimilated by fermentative microorganisms.Fermentation...

Production of Organic Acids

Production of Organic Acids

Lactic acid, an important organic acid extensively applied in food, pharmaceutical, and biodegradable polymer industries, is primarily produced via microbial fermentation. This method is favored over chemical synthesis due to its environmental sustainability and capacity for enantiomerically pure product formation. Among various microbial processes, the fermentation of starch-based substrates stands out due to the abundance and renewability of raw materials like corn and potatoes.Hydrolysis of...