Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

RNA-seq03:21

RNA-seq

10.0K
RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
10.0K
Ribosome Profiling02:24

Ribosome Profiling

3.5K
Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
3.5K
RACE - Rapid Amplification of cDNA Ends02:35

RACE - Rapid Amplification of cDNA Ends

6.4K
Rapid Amplification of cDNA Ends, or RACE, is one of the most effective methods to obtain a full-length cDNA from an mRNA sequence between a known internal region to the unknown sequence at the 5’ or 3’ end. The unknown region is cloned in the cDNA by a gene-specific primer that binds the known end, and a hybrid primer that attaches a predefined anchor sequence to the unknown end of the cDNA. The sequence in between is amplified by PCR with an anchor primer and a gene-specific...
6.4K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Spatially resolved profiling of extracellular vesicles in tissues with Spatial-EV-seq.

Nature biotechnology·2026
Same author

Cyclic transformation of stable/metastable nucleic acid structures enables dynamic monitoring of ATP in living cells.

Chemical science·2026
Same author

Dual-Spatially Confined Assembly of DNA Nanowall Stiffens Tumor Cells to Enhance Adoptive T-Cell Immunotherapy.

Journal of the American Chemical Society·2026
Same author

Tracing Tumor-Derived Extracellular Vesicle Matrix Metalloproteinase 14 Using Dual-Target Orthogonal Barcoding-Based Microscale Thermophoretic Assays.

ACS nano·2026
Same author

RENAL-CHIP: Rejection Evaluation via Non-Invasive Analysis of Circulating Podocytes With Herringbone-Chip Isolation Platform.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2026
Same author

Metabolic RNA Labeling-Enabled Time-Resolved Single-Cell RNA Sequencing.

Accounts of chemical research·2026

相关实验视频

Updated: Jul 5, 2025

Targeted RNA Sequencing Assay to Characterize Gene Expression and Genomic Alterations
11:52

Targeted RNA Sequencing Assay to Characterize Gene Expression and Genomic Alterations

Published on: August 4, 2016

10.4K

解码器-seq提高了空间RNA测序中的mRNA捕获效率.

Jiao Cao1, Zhong Zheng1, Di Sun1

  • 1Institute of Molecular Medicine, Shanghai Key Laboratory for Nucleic Acid Chemistry and Nanomedicine, Department of Urology, Renji Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.

Nature biotechnology
|January 16, 2024
PubMed
概括
此摘要是机器生成的。

使用Decoder-seq进行空间RNA测序可以提高mRNA检测的灵敏度和分辨率. 这项突破性的技术绘制了组织中的基因表达,有助于理解复杂的生物系统和疾病进展.

更多相关视频

Author Spotlight: AQRNA-seq Role in Mapping Small RNAs and Unraveling Protein Translation Mechanisms
05:12

Author Spotlight: AQRNA-seq Role in Mapping Small RNAs and Unraveling Protein Translation Mechanisms

Published on: February 2, 2024

760
3' End Sequencing Library Preparation with A-seq2
12:01

3' End Sequencing Library Preparation with A-seq2

Published on: October 10, 2017

10.6K

相关实验视频

Last Updated: Jul 5, 2025

Targeted RNA Sequencing Assay to Characterize Gene Expression and Genomic Alterations
11:52

Targeted RNA Sequencing Assay to Characterize Gene Expression and Genomic Alterations

Published on: August 4, 2016

10.4K
Author Spotlight: AQRNA-seq Role in Mapping Small RNAs and Unraveling Protein Translation Mechanisms
05:12

Author Spotlight: AQRNA-seq Role in Mapping Small RNAs and Unraveling Protein Translation Mechanisms

Published on: February 2, 2024

760
3' End Sequencing Library Preparation with A-seq2
12:01

3' End Sequencing Library Preparation with A-seq2

Published on: October 10, 2017

10.6K

科学领域:

  • 分子生物学分子生物学
  • 基因组学就是基因组学.
  • 生物技术是生物技术.

背景情况:

  • 高分辨率的空间转录组学经常与敏感的mRNA检测作斗争.
  • 现有的方法在全面的基因表达分析中,在平衡分辨率和灵敏度方面存在局限性.

研究的目的:

  • 介绍 Decoder-seq,一种用于空间RNA测序的新型树枝状DNA坐标条形编码方法.
  • 在mRNA检测中实现高灵敏度和高分辨率,用于先进的空间转录学.

主要方法:

  • 开发了Decoder-seq,将树枝状纳米基质与微流体坐标条形码结合起来.
  • 在空间数组上实现高DNA密度,大约是以前方法的十倍.
  • 解码器-seq的应用在小鼠的嗅觉球,海马和人类细胞癌.

主要成果:

  • 解码器-seq显著改善了小鼠嗅觉灯泡中低表达的嗅觉受体基因的检测.
  • 发现了两个嗅觉受体基因的独特层次丰富模式.
  • 鼠标海马的一个空间单细胞地图的构建,揭示了以树丰富的mRNAs.
  • 在人类细胞癌中剖析异质瘤微环境.
  • 识别与表皮细胞-介质细胞过渡相关的空间梯度表达基因,以预测预后.

结论:

  • 解码器-seq为敏感和高分辨率的空间转录学提供了一个强大的工具.
  • 该技术能够在复杂的组织和疾病环境中对基因表达进行详细的分析.
  • 解码器-seq有可能提高我们对神经生物学和癌症生物学的理解.