血清拉曼光谱:在实验性致癌过程中评估瘤负载变化
Priyanka A Jadhav1,2, Arti Hole1, Arvind Ingle1,2
1Chilakapati Laboratory, Advanced Centre for Treatment, Research and Education in Cancer (ACTREC), Tata Memorial Centre, Navi Mumbai, India.
Journal of biophotonics
|January 16, 2024
概括
血清拉曼光谱 (RS) 显示出口腔癌查的前景. 不同的瘤负载并没有混结果,支持其作为可靠的诊断工具的使用.
科学领域:
- 生物医学光谱学 生物医学光谱学
- 癌症的诊断 癌症的诊断
- 口腔瘤学 口腔瘤学
背景情况:
- 血清拉曼光谱 (RS) 已经显示出早期癌症检测的潜力.
- 了解影响RS精度的因素,如瘤负担,对于临床应用至关重要.
研究的目的:
- 调查低瘤负载 (LTL) 和高瘤负载 (HTL) 对血清RS的影响.
- 评估血清RS作为可靠的口腔癌查工具,无论瘤的阶段.
主要方法:
- 利用一个仓鼠口腔袋模型进行实验性口腔致癌.
- 收集和分析了随着时间的推移从控制,LTL和HTL组的血清拉曼光谱.
- 采用多变量分析,包括主要组件分析 (PCA),主要组件的线性差别分析 (PC-LDA) 和多变量曲线分辨率-交替最小平方 (MCR-ALS).
主要成果:
- 多变量分析显示,在所有时间点中,在LTL和HTL组之间存在错误分类.
- MCR-ALS在对照组和瘤携带组 (LTL和HTL) 之间发现了显著的生物分子差异.
- 包括蛋白质,脂质,血红素和核酸在内的MCR-ALS组件无法区分LTL和HTL.
结论:
- 血清拉曼光谱是一种强大的口腔癌查工具.
- 不同的瘤负载 (LTL和HTL) 不会混血清RS分析.
- 该技术对可靠的口腔癌诊断具有前景.
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