一个诱导亡的因子控制了在真菌相互作用期间的编程细胞死亡和乳酶表达
Junnan Fang1,2,3, Gang Zhou1,2,3, Huifang Zhao1,2,3
1School of Life Sciences, Anhui University, Hefei, 230601, Anhui, China.
Applied microbiology and biotechnology
|January 17, 2024
概括
在真菌中,线粒体CcAIF1触发编程细胞死亡 (PCD),并在真菌相互作用期间调节活性氧物种 (ROS). 过度表达增强了乳糖的产生,有助于防御反应和工业应用.
科学领域:
- 菌类学 菌类学是指菌类学.
- 细胞生物学 细胞生物学
- 生物化学 生物化学
背景情况:
- 编程细胞死亡 (PCD) 对真菌的平衡和应激反应至关重要.
- 诱导亡因子 (AIF) 同类物通过上调反应性氧物种 (ROS) 来调节PCD.
研究的目的:
- 确定和描述CcAIF1的作用,Coprinopsis cinerea中的线粒体AIF同类物.
- 研究CcAIF1在真菌与真菌相互作用,PCD,ROS调节和乳酶表达中的功能.
主要方法:
- 在Saccharomyces cerevisiae中CcAIF1的异构过度表达.
- 在Coprinopsis cinerea中,基因沉默和CcAIF1的过度表达.
- 对类似亡的PCD标记物的分析 (脂胺外化,DNA碎片化).
- 测量细胞内ROS度和乳糖酶基因/蛋白质表达.
主要成果:
- 异构的CcAIF1表达诱导了酵母中的类似apoptotic的PCD.
- 在C. cinerea与Gongronella sp.相互作用时,CcAIF1转录增加,与PCD相关.
- 沉默CcAIF1降低了ROS,亡,生长和分泌,而过度表达导致了相反的效果.
- CcAIF1调节乳糖酶表达 (Lcc9,Lcc8,Lcc13) 并增强乳糖酶分泌,特别是通过H2O2刺激.
结论:
- CcAIF1是ROS的线粒体调节者,在真菌-真菌相互作用中促进PCD和防御反应.
- CcAIF1在调节真菌生长,化和乳糖生产方面发挥着重要作用.
- CcAIF1是触发乳糖酶表达和分泌的关键因素,具有生物技术应用的潜力.
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