IS6家族插入序列促进了不同转移能力的等离子体之间的OctrA传播
Shihai Liu1,2,3, Xiaoxiao Yang1, Ruichao Li4
1State Key Laboratory of Environmental Aquatic Chemistry, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing, 100085, China.
Applied microbiology and biotechnology
|January 17, 2024
概括
插入序列 (ISs),特别是IS6家族基因,促进了OctrA抗生素耐药性基因在等离子体之间传播. 这种IS介导的移动性增加了OctrA传播的风险,特别是在来自人类,动物和环境来源的Enterococcus faecalis菌株中.
科学领域:
- 分子生物学分子生物学
- 遗传学 遗传学 是一个
- 微生物学 微生物学
背景情况:
- 等离子体是转移 optrA 基因的关键,赋予了对 oxazolidinones 和 phenicols 的耐药性.
- 插入序列 (ISs) 能够在细胞内调动由等离体传播的基因,但它们在等离体间基因传播中的作用尚不清楚.
- 了解IS介导的移动性对于跟踪抗生素耐药性基因传播至关重要.
研究的目的:
- 研究IS介导的细胞内移动性如何影响具有不同传输能力的等离子体中 optrA 基因的传播.
- 确定特定IS家族在OctrA传播中的流行率和作用.
- 评估来自不同来源的Enterococcus faecalis中 optrA 的传播潜力.
主要方法:
- 来自综合抗生素耐药性数据库的52种携带toprA的等离子体的基因组分析.
- 位于 optrA 基因附近的插入序列 (IS) 的识别和表征.
- 纳米孔测序和逆PCR用于确认Enterococcus faecalis中的基因传播机制.
主要成果:
- 在不同类型的等离子体中,IS6家族基因是在optrA附近发现的最常见的IS元素.
- IS6家族基因经常在可调动和结合性等离子体之间共享,这表明它们在基因转移中的作用.
- 来自人类,动物和环境来源的Enterococcus faecalis菌株 (肥处理废水) 携带了OctrA等离子体.
- 在肠球菌菌株中证实了IS6介导的OctrA从可调动到结合性等离子体的转移.
结论:
- 通过IS介导的细胞内移动性显著增强了等离子体传播的optRA基因的细胞间传输和传播风险.
- IS6家族基因在具有不同转移能力的等离子体中对OctrA的传播起着核心作用.
- 常规监测抗生素耐药性基因的遗传背景,如 optrA,对于公共卫生至关重要.
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