在Alternaria中转录因子Cmr1同类的积极选择和功能多样化
Chaodong Qiu1, Zhenyu Liu2,3
1Department of Plant Pathology, School of Plant Protection, Anhui Agricultural University, Hefei, Anhui, 230036, China.
Applied microbiology and biotechnology
|January 17, 2024
概括
在Alternaria真菌中转录因子Cmr1同类物在积极选择下进化,影响黑色素的产生,致病性和应激反应. 这表明Cmr1可以作为Alternaria物种的分类学标记.
科学领域:
- 植物病理学 植物病理学
- 菌类学 菌类学是指菌类学.
- 分子进化分子进化
背景情况:
- 转录因子Cmr1调节了真菌病原体中的1,8-二氧化纳乙烯 (DHN) - 黑色素生物合成.
- 被称为"黑真菌"的Alternaria物种拥有丰富的DHN-黑色素,并导致重大植物疾病.
- 在Alternaria中Cmr1同类的进化历史和功能作用尚不清楚.
研究的目的:
- 研究Cmr1同类物种在Alternaria属中的演化和功能.
- 确定这些转录因子在病原性和形态学的关键进化压力和功能作用.
- 评估Cmr1作为Alternaria.的分类学标记物的潜力.
主要方法:
- 从42种Alternaria物种中鉴定和对42种Cmr1同类物种的基因分析.
- 分子进化分析,包括积极选择检测.
- 来自Alternaria solani的Asmr1的功能性特征,包括转录激活试验和基因删除实验.
主要成果:
- 确定了42种Cmr1同类物,分为5个分类和3个分支,在3个氨基酸位点有积极选择的证据.
- 在Alternaria solani的同类物Asmr1中,表现出转录激活,在感染期间被上调,其删除损害了黑色素含量,致病性和应激耐受性.
- 在不同的Alternaria solani分离物中观察到Asmr1的序列保存.
结论:
- 积极选择和功能分歧塑造了Alternaria中Cmr1同类物种的进化.
- 在Alternaria solani中,Asmr1在子发育,黑色素生物合成,致病性和应激反应中起着至关重要的作用.
- Cmr1同类体代表了Alternaria属的潜在分类学标记.
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