亚斯科和巴西迪奥基菌 O-基转移酶的并行进化
Tim Schäfer1,2, Fabian Haun1,2, Markus Gressler1,2
1Institute of Pharmacy, Friedrich Schiller University, Winzerlaer Strasse 2, 07745 Jena, Germany.
Journal of natural products
|January 17, 2024
概括
研究人员特征了一种来自Laccaria proxima的新型prenyltransferase (PT),LpTyrPT. 这种酶对氨酸和氨酸进行了prenylates,为真菌的二次新陈代谢和prenyltransferases的演变提供了新的见解.
科学领域:
- 生物化学 生物化学
- 菌类学 菌类学是指菌类学.
- 酶学 是一种酶学.
背景情况:
- 甲基转移酶 (PTs) 是合成植物,细菌和真菌中有价值化合物的关键酶.
- 虽然许多化化合物已知来自Basidiomycota (形成的真菌),但涉及的特定PTs仍然在很大程度上没有特征.
研究的目的:
- 从生物化学上表征一种来自基底菌 Laccaria proxima 的新型 prenyltransferase.
- 为了研究这种酶的基质特异性和反应机制.
主要方法:
- 生物化学特征的二甲基利三酸合成酶 (DMATS) 类似的酶LpTyrPT.
- 在真菌种群中对prenyltransferases的遗传学分析.
主要成果:
- LpTyrPT被确定为一种能够同时进行O-和C-prenylation的酶.
- 该酶将二甲基基转移到L-tyrosine在para-O位置和L-tryptophan在C-7位置.
- 这代表了来自Basidiomycota属的第一个描述的L-氨酸前转移酶.
结论:
- LpTyrPT是一种新型酶,在L-氨酸和L-三上具有双重O-和C-prenylating活性.
- 贝西迪奥米塞特L-氨酸PTs似乎已经独立地从他们的阿斯科米塞特对应物进化.
- 这些发现表明,参与真菌二次代谢中的化合物生物合成的 PT 具有潜在的进化起源.
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