描述与储存谷物相关的阿斯伯吉卢斯部分Flavi的特征
Eman G A M El-Dawy1,2, Youssuf A Gherbawy3,4, Mohamed A Hussein3,4
1Botany and Microbiology Department, Faculty of Science, South Valley University, Qena, Egypt. eman.mohamed11@sci.svu.edu.eg.
Mycotoxin research
|January 17, 2024
概括
气候变化增加了谷物中的 aflatoxin污染. 这项研究确定了大麦和玉米中的Aspergillus物种,发现了非洲毒素的生产者,并测试了一种生物控制剂Trichoderma viride,以减少污染.
科学领域:
- 农业科学 农业科学
- 菌类学 菌类学是指菌类学.
- 食品安全 食品安全
背景情况:
- 全球气温上升和干旱条件与谷物谷物中Aspergillus部分Flavi和亚毒素的流行率增加有关.
- 准确识别真菌污染物对于评估食品供应中的微生物风险至关重要.
研究的目的:
- 来自埃及Qena的大麦和玉米粒中的Flavi部分的Aspergillus物种的特征.
- 调查阿弗拉托克辛生物合成基因的存在及其与阿弗拉托克辛生产的相关性.
- 为了评估Trichoderma viride作为生物控制剂对抗 aflatoxin污染的疗效.
主要方法:
- 使用形态学和分子技术,从大麦和玉米样本中识别出23个阿斯伯吉路斯单独体.
- 分析了阿弗拉托克辛生物合成基因 (aflR,aflD,aflM) 与阿弗拉托克辛和阿弗拉托克辛B1总产量的关系.
- 评估了Trichoderma viride对抗Aspergillus flavus的对抗性活性,并测量了其对治疗种子中总亚毒素的影响.
主要成果:
- 鉴定出的阿斯伯吉卢斯物种包括A. aflatoxiformans (4),A. flavus (18) 和A. parasiticus (1).
- 十五个隔离物产生了总的亚毒素 (0.1-240 ppm),大多数情况下,生产与aflR1/aflR2基因有关.
- 特里科德尔马维里德 (Trichoderma viride) 显示出对抗性活性 (31.3%),并降低了对待的大麦和玉米种子中的总 Афлатоксин.
结论:
- 形态和分子方法有效地识别了埃及谷物中的阿斯伯吉洛斯 (Aspergillus) 部分Flavi物种.
- 特定的亚拉托克辛生物合成基因的存在与亚拉托克辛的产生有关,尽管其他因素也可能参与其中.
- 特里科德尔马维里德 (Trichoderma viride) 显示出作为一种生物控制剂的潜力,以减轻谷物谷物中的亚拉托克辛污染.
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