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Updated: Jul 5, 2025

A Fluorescence Fluctuation Spectroscopy Assay of Protein-Protein Interactions at Cell-Cell Contacts
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A Fluorescence Fluctuation Spectroscopy Assay of Protein-Protein Interactions at Cell-Cell Contacts

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在液态-液态相分离过程中检测蛋白质-蛋白质相互作用,使用化蛋白质传感器.

Yanan Huang1, Junlin Chen1, Chia-Heng Hsiung1

  • 1Department of Chemistry, Research Center for Industries of the Future, Westlake University, Hangzhou 310030, Zhejiang, China.

Molecular biology of the cell
|January 17, 2024
PubMed
概括

分化依赖光蛋白 (ddFPs) 能够在液体-液体相分离 (LLPS) 过程中检测蛋白相互作用. 这种化学生物学方法揭示了RNARNA.

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Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
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