聚3-基酸盐) 通过使用Bacillus megateriumATCC 14581从纤维素废物中生产
Lacrimioara Senila1, Emese Gál2, Eniko Kovacs1,3
1Research Institute for Analytical Instrumentation Subsidiary, National Institute for Research and Development of Optoelectronics Bucharest INOE 2000, 67 Donath Street, 400293 Cluj-Napoca, Romania.
Polymers
|January 17, 2024
概括
这项研究优化了基纤维素生物质的聚3-基酸盐 (PHB) 生产. 微波预处理和氨脱显著改善了使用Bacillus megaterium的PHB产量.
科学领域:
- 生物技术和应用微生物学
- 聚合物科学与工程 聚合物科学与工程
- 可持续材料科学 可持续材料科学
背景情况:
- 细胞生物质是生物聚合物生产的丰富,可再生的资源.
- 效率高的纤维素转化为有价值的产品,如聚3-基酸盐 (PHB) 仍然是一个挑战.
- 优化预处理和水解对于提高发酵产量至关重要.
研究的目的:
- 开发一种优化工艺,从纤维纤维素生物质中生产聚3-基酸盐 (PHB).
- 评估微波辐射,氨脱和酶化水解对PHB产量的影响.
- 用先进的分析技术来描述合成的PHB.
主要方法:
- 使用微波辐射 (180-220 °C 10-30 分钟) 进行红纤维素生物质预处理.
- 通过中央复合材料设计 (CCD) 和响应表面方法 (RSM) 优化预处理条件.
- 使用Bacillus megateriumATCC 14581.1.进行氨酸脱,酶化水解和随后的发酵.
- 使用NMR (1H, 13C),EI-MS,XRD,FT-IR和TGA进行PHB的表征.
主要成果:
- 使用RSM确定了最佳的微波预处理条件.
- 氨脱显著增加了生物质水解产量,从70.2%提高到91.4%.
- 巨杆菌有效地从优化的基纤维素化物中合成PHB.
- 鉴定证实了所产生的PHB的部分晶体性质.
结论:
- 集成的微波预处理,氨脱和酶性水解是一种有效的基纤维素生物质价值化策略.
- 这种优化的工艺提高了适合微生物PHB生产的碳基板的产量.
- 这项研究表明,从可再生的纤维纤维素资源中实现可持续的聚3-基酸盐合成的可行途径.
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