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编辑转运器基因以减少大米中的积累

Xuejie Xu¹, Sheng-Kai Sun¹, Wenwen Zhang¹

¹State Key Laboratory of Crop Genetics and Germplasm Enhancement and Utilization, College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing210095, China.

Environmental science & technology

|January 17, 2024

概括

在大米中编辑OsLsi2基因可以显著降低谷物中的积累量,高达63%,而不会影响谷物产量. 这种基因改造为更安全的米生产和改善食品安全提供了有前途的战略.

科学领域:

植物分子生物学植物分子生物学
农业科学农业科学
食品安全食品安全

背景情况:

米的消费是无机 (As) 暴露的主要来源.
减少大米谷物中的积累对于公共卫生和食品安全至关重要.
在土中占主导地位的形式化物[As(III) ]通过载体OsLsi1和OsLsi2.2被米所吸收.

研究的目的:

调查编辑OsLsi1或OsLsi2基因在减少大米粒中积累的有效性.
评估这些基因改造对大米谷物产量的影响.

主要方法:

利用CRISPR-Cas9技术编辑OsLsi1和OsLsi2基因的促进子和编码序列,产生27个突变.
在水培和田实验中评估了 (Si) 和 (As) 的吸收和积累.
分析了突变对粒中酸和二甲基酸盐 (DMA) 积累的影响.

主要成果:

编辑OsLsi1促进体并没有改变As(III) 吸收或谷物As度.
在OsLsi1和OsLsi2编码序列中的突变减少了Si和As (III) 的吸收,并在中积累Si.
OsLsi2突变对谷物总度显著降低 (高达63%),主要影响无机.
在OsLsi2突变中,谷物产量与野生类型大米相比仍然相当.

结论:

编辑OsLsi2基因是一种有效的策略,可以减少在米粒中的无机积累.
这种方法显著提高了食品安全,而不会损害谷物的基本产量.
准OsLsi2为开发低米品种提供了一种可行的方法.

关键词:

这就是CRISPR-Cas9的特征.美国的奥斯陆西1 亚是一种.基因编辑基因编辑米米饭米饭米饭米饭.

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