培氨酸涂层和酸盐涂层甲酸脂质体的制备:关于特征,稳定性,消化和释放行为的研究
Lingling Su1, Dandan Zhao1, Qiong Huang2
1College of Food Science & Biology, Hebei University of Science & Technology, Shijiazhuang 050000, China.
International journal of biological macromolecules
|January 17, 2024
概括
这项研究使用挤出和超声波增强了脂质体制备,提高了封装效率和Phytoecdysones (PhGs) 的生物可用性. pectin和chitosan涂层提高了消化系统的稳定性,以改善PhG的传递.
科学领域:
- 制药科学 制药科学
- 材料科学 材料科学 材料科学
- 生物技术是生物技术.
背景情况:
- 脂质体是关键的药物输送系统.
- 提高像Phytoecdysones (PhGs) 这样的活性化合物的封装效率和生物可用性仍然是一个挑战.
- 需要新的制备技术来提高脂质体的性能.
研究的目的:
- 研究挤出和超声波对脂质体物理化学性质的影响.
- 为了评估Phytoecdysone脂质体 (PhGs唇),素涂层的PhGs唇 (P-唇) 和酸盐涂层的PhGs唇 (C-唇) 的质量.
- 评估涂层对脂质体稳定性和生物可用性的影响.
主要方法:
- 通过乙醇注射与挤出超声波相结合而制备的脂质体.
- 使用颗粒大小分析进行表征,封装效率 (EE) 测量.
- 显微镜 (TEM),光谱镜 (FTIR) 和热分析 (DSC).
- 在体外细胞实验中评估生物可用性和消化稳定性.
主要成果:
- 与单个方法相比,挤出超声波方法显著增加了EE (8%和18%更高).
- 通过表征技术确认成功制备纳米粒子.
- 证据表明PhG和脂之间的键,以及涂层和脂质体之间的相互作用.
- 改善了被涂层脂质体的生物可用性和显著增强的消化稳定性 (在模拟的肠液中保持率提高了25%).
结论:
- 联合挤出-超声波是制备高质量的PhG脂质体的有效方法.
- pectin和chitosan涂层增强了PhGs的消化稳定性和生物可用性.
- 这些修改后的脂质体显示出改善药物输送应用的前景.
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