通过合成生物学方法扩大烯的结构多样性
Rong Chen1, Ming Wang2, Jay D Keasling3
1Key Laboratory of Elemene Class Anti-Cancer Chinese Medicine of Zhejiang Province, School of Pharmacy, School of Public Health, Hangzhou Normal University, Hangzhou 310000, China; Joint BioEnergy Institute, Emeryville, CA 94608, USA.
Trends in biotechnology
|January 17, 2024
概括
本综述探讨了创造各种非自然类的新方法. 这些方法扩大了结构可能性,超越了天然产品的限制,使新的应用成为可能.
科学领域:
- 生物化学 生化学
- 合成生物学 合成生物学
- 自然产品化学 自然产品化学
背景情况:
- 体表现出显著的化学多样性和生物活动,但它们的结构通常受到五碳 (C5) 异烯单元的限制.
- 现有的类结构是从可互换的C5单元衍生出来的,这限制了天然产品的范围.
研究的目的:
- 系统地审查新出现的扩大类结构多样性的策略.
- 要突出产生非正规的类构建块和非自然类的方法.
主要方法:
- 通过联合表达甲酸盐 (MVA) 途径和C-甲基转移酶 (C-MTs) 的组合生物合成.
- 在非正规的前体合成中利用类旋酸盐 (LMVA) 途径.
- 化学生物合成使用法纳西二酸盐 (FPP) 类似物.
- 由人工金属酶 (ArM) 催化的烯环化.
主要成果:
- 展示产生化物合成非正规构件的途径.
- 使用工程生物合成路径和合成化学制造非自然类的创造.
- 讨论加速烯生物合成的先进技术.
结论:
- 新的生物合成和化学生物学方法显著扩大了类的结构多样性.
- 这些进步为制造各种类和衍生品提供了有价值的参考.
- 突出了这些非自然类的扩大潜在应用.
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