综合转录组和代谢组分析揭示了多珀和坦羊的品种特异性调节机制
Yuhao Ma1, Ganxian Cai1, Jianfei Chen1
1Key Laboratory of Animal Genetics, Breeding, and Reproduction of the Ministry of Agriculture and Beijing Key Laboratory of Animal Genetic Improvement, China Agricultural University, Beijing, 100193, China.
BMC genomics
|January 17, 2024
概括
羊通过氨基酸合成表现出快速增长,而羊通过增强胆汁运输和胆固醇调节表现出改善的肉质. 这项研究将品种特征与特定的代谢途径和基因表达联系起来.
科学领域:
- 动物基因组学和新陈代谢
- 比较生理学比较生理学
- 肠道微生物组研究研究
背景情况:
- 羊以快速生长而闻名;棕色羊以肉质而闻名.
- 肠道微生物群影响品种特征,但器官特异性相关性尚不清楚.
- 肠道代谢网络可以影响动物的表现和经济价值.
研究的目的:
- 为了研究多珀羊和坦羊之间的代谢和转录基因差异.
- 识别关键的基因和代谢物,这些基因和代谢物有助于特定品种的特征,如生长和肉质.
- 阐明胃肠道和外周器官功能之间的相关性.
主要方法:
- 对大脑,肝脏,骨肌肉和肠道组织的转录组分析.
- 对后肠含量中差异表达基因 (DEG) 和差异积累代谢物 (DAM) 的分析.
- 多组织转录组和肠道代谢组数据的综合分析.
主要成果:
- 在Dorper和Tan羊群中确定了3750个DEG和200个DAM.
- 沉睡羊表现出高调的脂质氧化和氨基酸合成通路 (例如PPARGC1A,LPL,PHGDH).
- 棕色羊表现出高调节的胆汁运输和胆固醇平衡途径 (例如,BSEP,LDLR,ACHE).
结论:
- 品种特有的特征源于不同的生理调节机制.
- 羊的生长与甘氨酸,氨酸,氨酸和 purin/pyrimidine 的新陈代谢有关.
- 羊的肉质量与加速胆汁运输和骨肌肉中的胆固醇调节有关.
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