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精制和照明乙烯基Eubacterium菌株进行重新分类和代谢工程

Maximilian Flaiz^1,2, Anja Poehlein³, Wiebke Wilhelm⁴

¹Laboratory of Microbiology, Wageningen University and Research, Wageningen, The Netherlands. maximilian.flaiz@wur.nl.

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概括

包括E. limosum在内的Eubacterium菌株是优秀的生物催化剂,可以将C1基质转化为像丁酸盐这样的有价值产品. 研究人员成功地通过基因工程改造了11种菌株,从而实现了新的生物技术应用.

科学领域:

微生物学微生物学
合成生物学合成生物学
生物技术是生物技术.

背景情况:

欧细菌属含有多种能够发酵C1基质的乙烯基菌株.
尤巴菌 (Eubacterium limosum) 和相关物种在C1气/液体发酵和酸盐生产方面显著.
E. limosum 的已知的新陈代谢和遗传可访问性使其成为一个有前途的工业生物催化剂.

研究的目的:

研究E. limosum及其近亲的基因组,遗传学和生理特征.
确定负责C1基质利用和链延长的遗传元素.
为工业应用建立可靠的基因工程协议.

主要方法:

11个Eubacterium菌株的基因组测序和对比分析.
遗传学分析以确定不同的类 (E. limosum,E. callanderi,E. maltosivorans).
增长实验以评估甲醇转化和产品形成 (乙酸盐,丁酸盐,六酸盐).
为基因转换开发和应用一个协调的电穿孔协议.
使用光激活和吸收转移标签 (FAST) 作为通过流细胞计进行基因工程验证的报告员.

主要成果:

十一个Eubacteriaum菌株被分为三个不同的分类:E. limosum,E. callanderi和E. maltosivorans.
在所有分析的菌株中确定了用于甲醇利用和链延长的基因集群.
证明所有三种类型的菌株可以通过逆β氧化将甲醇转化为酸盐,丁酸盐和六酸盐.
通过使用协调的电穿孔协议和FAST报告员,成功地基因改造了八种Eubacteria菌株.

结论:

E. limosum,E. callanderi和E. maltosivorans是无氧C1基板生物转化的主要候选者.
一个标准化的电穿孔协议使得基因工程能够跨越多个Eubacterium菌株.
快速作为一个有效的光记者,用于表征工程 Eubacterium.
更新了11个菌株的分类,并将其分为不同的分类,并建议修改物种描述以实现数据库.

关键词:

乙原剂是一种原剂.无气体生物无气体生物欧洲细菌 (Eubacterium) 是一种细菌.很快,非常快,非常快.光是一种光效应.六酸盐的使用方法甲醇是甲醇中的一种.一个叫Anderieri的电话.一个清洁的汽车.马尔托西沃兰斯 (马尔托西沃兰斯) 是一种

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