Hsa_circ_0008833 通过诱导支气管上皮细胞中的 pyroptosis 来促进 COPD 的进展
Tian Xie1, Zehua Yang1, Shaojing Xian1
1Department of Pulmonary and Critical Care Medicine, Hainan affiliated Hospital of Hainan Medical University, Hainan General Hospital, Haikou, Hainan, China.
像has-circ-0008833这样的循环RNAs (circRNAs) 在慢性阻塞性肺病 (COPD) 中被上调. 这种circRNA及其编码的在支气管细胞中促进pyroptosis,这表明它在COPD进展中的作用.
科学领域:
- 分子生物学分子生物学
- 细胞生物学 细胞生物学
- 呼吸系统医学 呼吸系统医学
背景情况:
- 慢性阻塞性肺病 (COPD) 是一种普遍存在的呼吸道疾病,其特征是炎症.
- 炎症性细胞死亡途径 - - 热,涉及卡斯帕-1和炎症细胞.
- 循环RNAs (circRNAs) 正在成为各种人类疾病的关键参与者.
研究的目的:
- 为了研究COPD进展中的circRNA概况.
- 为了确定与COPD发展相关的特定circRNAs.
- 阐明has-circ-0008833及其编码蛋白在COPD病变发生过程中的作用.
主要方法:
- 在COPD患者的外周血液单核细胞 (PBMC) 中使用微阵列进行circRNA表达概况.
- 在16HBE细胞中评估has-circ-0008833的蛋白质编码潜力和功能分析.
- 西部斑点分析以评估与热死相关的标记物 (Caspase-1,IL-18,IL-1β,NLRP3,ASC,裂开的GSDMD).
主要成果:
- has-circ-0008833表达在COPD患者的PBMC中显著增加.
- 发现has-circ-0008833编码了一个功能性蛋白质,circ-0008833-57aa.
- 在16HBE细胞中,has-circ-0008833和circ-0008833-57aa都抑制了细胞增殖和诱导细胞死亡.
- 这些分子上调了关键的热灭菌标记物,包括Caspase-1和分裂的GSDMD.
结论:
- 上调的circ-0008833可能会导致COPD的进展.
- 该机制涉及在支气管上皮细胞中诱导热致死.
- 这种效应是通过编码为circ-0008833.33的57氨基酸介导的.
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