相关实验视频
Updated: Jul 5, 2025
13:00
Engineering Antiviral Agents via Surface Plasmon Resonance
Published on: June 14, 2022
2.4K
向蛋白质-蛋白质相互作用接口与SARS-CoV-2中的抗病毒N蛋白抑制剂
Jhen-Yi Hong1, Shih-Chao Lin2, Kylene Kehn-Hall3
1Institute of Genomics and Bioinformatics and Department of Life Sciences, National Chung Hsing University, Taichung, Taiwan.
Biophysical journal
|January 18, 2024
概括
抗病毒化合物5-氧胺 (P3) 通过诱导非原生相互作用来抑制病毒功能,有效地向包括SARS-CoV-2在内的冠状病毒的核体 (N) 蛋白N-终端域 (N-NTD).
科学领域:
- 病毒学 病毒学
- 结构生物学 结构生物学
- 药物发现 药物发现 药物发现
背景情况:
- 冠状病毒 (CoV) 给全球健康和经济带来了重大挑战.
- 核体 (N) 蛋白的N-终端域 (N-NTD) 对CoV复制至关重要.
- 5 - 基洛克西格拉 (P3) 通过向N-NTD,表现出针对Covs的广泛抗病毒活性.
研究的目的:
- 阐明P3广泛的抗CoV活性机制.
- 为了确定SARS-CoV-2 N-NTD:P3复合体的晶体结构.
- 为了比较SARS-CoV-2 N-NTD与MERS-CoV N-NTD中的P3结合.
主要方法:
- 进行X射线晶体学以确定SARS-CoV-2 N-NTD:P3复合体的结构.
- 在N-NTD疏水口袋内对P3结合的结构分析.
- 在不同CoV之间对P3-N-NTD接口进行比较分析.
主要成果:
- 通过疏水性相互作用,P3与SARS-CoV-2 N-NTD二元体结合.
- 与MERS-CoV N-NTD相比,P3在SARS-CoV-2 N-NTD中采用了相反的方向.
- 在SARS-CoV-2 N-NTD口袋是更多的疏水性,容纳P3结合有效.
结论:
- 尽管有序列变异,但P3通过疏水相互作用有效地与各种CoVN-NTD结合.
- 这种结合会诱导非原生蛋白与蛋白相互作用 (PPI),损害N蛋白的功能.
- 由于P3能够容纳不同的疏水口袋,因此它具有针对冠状病毒的广泛抗病毒功效.
相关概念视频
02:04Protein-protein Interfaces
12.5K
Many proteins form complexes to carry out their functions, making protein-protein interactions (PPIs) essential for an organism's survival. Most PPIs are stabilized by numerous weak noncovalent chemical forces. The physical shape of the interfaces determines the way two proteins interact. Many globular proteins have closely-matching shapes on their surfaces, which form a large number of weak bonds. Additionally, many PPIs occur between two helices or between a surface cleft and a...
12.5K
02:04Protein-Protein Interfaces
3.8K
3.8K
02:50Conjugated Proteins
18.3K
Simple proteins and protein complexes contain only amino acids. In contrast, many other proteins, called conjugated proteins, covalently bond with non-protein moieties.
Nucleoproteins are protein complexes that contain nucleic acids, categorized as deoxyribonucleoproteins (DNPs) or ribonucleoproteins (RNPs) respectively. The nucleosome is a typical example of a DNP where nuclear DNA is associated with histone proteins. The major antigen for the Covid-19 virus SARS-CoV is an RNP that is critical...
Nucleoproteins are protein complexes that contain nucleic acids, categorized as deoxyribonucleoproteins (DNPs) or ribonucleoproteins (RNPs) respectively. The nucleosome is a typical example of a DNP where nuclear DNA is associated with histone proteins. The major antigen for the Covid-19 virus SARS-CoV is an RNP that is critical...
18.3K
02:26Protein Networks
4.0K
An organism can have thousands of different proteins, and these proteins must cooperate to ensure the health of an organism. Proteins bind to other proteins and form complexes to carry out their functions. Many proteins interact with multiple other proteins creating a complex network of protein interactions.
These interactions can be represented through maps depicting protein-protein interaction networks, represented as nodes and edges. Nodes are circles that are representative of a protein,...
These interactions can be represented through maps depicting protein-protein interaction networks, represented as nodes and edges. Nodes are circles that are representative of a protein,...
4.0K