表面修饰对个体上转化纳米粒子发光效应的影响
Huan Ling1, Daoming Guan1, Rongrong Wen1
1Department of Chemistry and Shanghai Key Laboratory of Molecular Catalysis and Innovative Materials, Fudan University, Shanghai, 200438, China.
Small (Weinheim an der Bergstrasse, Germany)
|January 18, 2024
概括
表面修改改善了单分子成像中使用的胺化上转化纳米粒子 (UCNPs) 的水分散性. DSPE-PEG涂层在水中提供最明亮的单颗粒发光.
科学领域:
- 纳米技术 纳米技术
- 材料科学 材料科学 材料科学
- 生物光子学 生物光子学
背景情况:
- 由于其光稳定性和低自发光度,在单分子成像中具有价值的Lanthanide-doped upconversion纳米粒子 (UCNPs).
- 像油酸这样的疏水性配体限制了UCNP的水分散性,阻碍了生物应用.
研究的目的:
- 调查各种表面修饰策略对水性环境中UCNP单颗粒上转换发光的影响.
- 确定最佳的表面化学物质,以提高UCNP在水中的性能,用于成像应用.
主要方法:
- 合成的UCNP通过配体交换 (IR806),化学处理 (HCl,NOBF4),涂层 (SiO2,mSiO2) 和聚合物自组装 (DSPE-PEG,F127) 进行了表面修饰.
- 测量了单颗粒上升转换的发光强度和灭,用于水中的每个修改策略.
- 在不同辐射强度下分析了红色到绿色的排放比率.
主要成果:
- NOBF4和DSPE-PEG修改显示了最小的发光灭 (3%和8%).
- 由于晶格的破坏,HCl处理严重灭光发 (84%).
- DSPE-PEG涂层的UCNP在水中表现出最高的单颗粒发光,保留了48%的原始辐射.
- 红色的辐射占主导地位,而绿色的辐射占主导地位,该比率随着辐射强度的增加而增加.
结论:
- 表面化学显著影响UCNP发光和单颗粒水平上的水分散性.
- DSPE-PEG涂层是一种高效的策略,可以实现用于成像的明亮,水可分散的UCNP.
- 了解表面效应对于优化UCNP用于生物成像和其他应用至关重要.
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