相关实验视频

Updated: Jul 5, 2025

CRISPR/Cas9 Editing of the C. elegans rbm-3.2 Gene using the dpy-10 Co-CRISPR Screening Marker and Assembled Ribonucleoprotein Complexes.

CRISPR/Cas9 Editing of the C. elegans rbm-3.2 Gene using the dpy-10 Co-CRISPR Screening Marker and Assembled Ribonucleoprotein Complexes.

Published on: December 11, 2020

一个有针对性的CRISPR-Cas9介导的F0屏幕识别了参与建立肠道神经系统的基因

Rodrigo Moreno-Campos^1,2, Eileen W Singleton^1,2, Rosa A Uribe^1,2

¹Biosciences Department, Rice University, Houston, Texas, 77005, U.S.A.

bioRxiv : the preprint server for biology

|January 18, 2024

概括

这项研究在斑马鱼中引入了一种新的CRISPR屏幕,以发现调节肠道神经系统 (ENS) 发育的基因. 研究结果显示,阿片类受体是ENS形成和神经化学编码的关键参与者.

科学领域:

发育生物学是发展生物学.
神经科学是一个神经科学.
遗传学遗传学是一个

背景情况:

脊椎动物的肠道神经系统 (ENS) 来自肠道神经细胞 (ENCCs),控制胃肠功能和肠-大脑轴通信.
在ENCC的发展中出现的障碍会导致诸如赫施普隆病 (Hirschsprung disease,HSCR) 这样的疾病,其特点是肠道内缺少ENS.
了解ENS发育对于解决先天性胃肠道疾病至关重要.

结论:

该研究提出了一种有效的CRISPR查方法,用于调查ENS发育.
这些发现扩大了对神经系统结构的遗传调节者的知识.
阿片类受体被认为是ENS形成和功能中的重要因素.

关键词:

这是一个CRISPR屏幕.肠道神经系统肠道神经系统肠道神经系统的发育和发展肠道神经元的神经元这是一种阿片类阿片类药物.斑马鱼是一种斑马鱼.

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CRISPR

CRISPR

Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...

CRISPR and crRNAs

CRISPR and crRNAs

Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...