铜离子纳米颗粒的绿色合成功能化与拉姆诺利皮德作为潜在的抗菌剂致病性细菌的细菌
Fera Faridatul Habibah1, Wa Ode Sri Rizki1, Atthar Luqman Ivansyah2
1Biochemistry Research Division, Faculty of Mathematics and Natural Sciences, Institute Technology Bandung, Bandung, Indonesia.
Heliyon
|January 18, 2024
概括
环保的铜I-脂纳米颗粒 (CuI-Rl Nps) 显示出卓越的抗菌活性. 这些新型纳米颗粒提供了一种强大且具有成本效益的解决方案,可以对抗阳性和阴性细菌.
科学领域:
- 材料科学 材料科学 材料科学
- 纳米技术纳米技术
- 微生物学 微生物学
背景情况:
- 基于铜的纳米颗粒具有广泛的抗菌特性,为其他基于金属的纳米颗粒提供了具有成本效益的替代品.
- 使用可生物降解和无毒的生物表面活性剂开发环保的纳米粒子是一个活跃的研究领域.
研究的目的:
- 为了合成和表征环保的铜(I) - 拉姆诺化物纳米颗粒 (Cu(I) - Rl Nps).
- 为了评估Cu(I) -Rl Nps对格拉姆阳性和格拉姆阴性细菌的抗菌功效.
主要方法:
- 使用共同降水方法制备Cu(I) -Rl Nps.
- 结构分析是使用里埃变换红外光谱 (FTIR) 和X射线衍射 (XRD) 进行的.
- 通过传输电子显微镜 (TEM),粒子大小分析 (PSA) 和泽塔潜力分析 (ZPA) 评估了形态和表面特性.
- 通过最小抑制度 (MIC) 和最小杀菌度 (MBC) 试验来确定抗菌活性.
主要成果:
- FTIR和XRD证实了Cu(I) -Rl Nps的成功合成,由rhamnolipid和Cu(I) 离子之间的离子和共价协调键的形成表明.
- TEM,PSA和ZPA揭示了直径范围为141.7536.3nm的球形纳米粒子,表面电荷为-30 mV.
- 与基于铜的纳米颗粒,自由的Cu (I) 离子和脂相比,Cu (I) -Rl Nps表现出显著增强的抗菌活性.
- 对于*Bacillus subtilis*,Cu(I) -Rl Nps的MIC/MBC值为19/19μg/mL,明显低于对照组.
- 对于大肠杆菌,Cu (I) -Rl Nps的MIC/MBC值为78/78μg/mL,也显示出更高的疗效.
结论:
- 通过共沉合成Cu(I) -Rl Nps是可行的,并产生稳定的纳米粒子.
- (I) -Rl Nps增强的抗菌活性归因于 (I) 离子和脂之间的协同作用.
- Cu(I) -Rl Nps 是一个有前途的环保和高效的抗菌剂.
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