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改变RNA病毒检测的范式:通过单分子数字ELISA集成核酸测试和免疫测试

Zhiyong Wang1, Pei Wei1

  • 1Department of Immunology, Zunyi Medical University, Zhuhai, China.

Frontiers in immunology
|January 18, 2024
PubMed
概括

本综述讨论了RNA病毒的威胁和检测方法. 结合核酸测试与免疫学测试,特别是数字ELISA,提供了有希望的实时,大规模的RNA病毒检测能力.

关键词:
RNA病毒的RNA病毒数字ELISA是一个数字ELISA.免疫测试是一种免疫测试.核酸测试核酸测试试验单个分子检测检测单个分子检测

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Enzyme-Linked Immunosorbent Assay01:33

Enzyme-Linked Immunosorbent Assay

In 1971, Peter Perlman and Eva Engvall developed an Enzyme-linked immunosorbent assay (ELISA or EIA). ELISA differs from western blot in that the assays are conducted in microtiter plates or in vivo rather than on an absorbent membrane.
There are many different types of ELISAs, but they all involve an antibody molecule whose constant region binds an enzyme, leaving the variable region free to bind its specific antigen.  Enzyme-substrate reaction allows the antigen to be visualized or quantified.