在Saccharomyces cerevisiae静止退出期间的肋骨蛋白质组重塑
Clara A Solari1, María Clara Ortolá Martínez1, Juan M Fernandez1
1Universidad de Buenos Aires, Facultad de Ciencias Exactas y Naturales, Departamento de Química Biológica, Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales-Consejo Nacional de Investigaciones Científicas y Técnicas (IQUIBICEN-CONICET), Buenos Aires, Argentina.
iScience
|January 18, 2024
概括
细胞静止涉及具有特殊功能的异质核糖体. 这项研究确定了核糖体蛋白和辅助蛋白,揭示了它们在静止期间对转化活动和细胞过程的影响.
科学领域:
- 分子生物学分子生物学
- 细胞生物学 细胞生物学
- 生物化学 生物化学
背景情况:
- 静止状态是一种基本的细胞状况,对于理解细胞循环调节,衰老和对刺激的反应至关重要.
- 众所周知,异质核糖体具有专门的翻译功能,使细胞能够适应其蛋白质组.
- 麦芽菌 (Saccharomyces cerevisiae) 作为一个强大的模型生物来研究这些细胞过程.
研究的目的:
- 在Saccharomyces cerevisiae的细胞静止期间全面识别和描述真核80S核糖体的蛋白质成分.
- 研究已识别的核糖体蛋白 (RP) 和与静止细胞中的核糖体相关的辅助蛋白的功能作用.
- 确定核糖体构成和固体几何学在不同的生长条件下以及不同核糖体分数 (单体与多体) 中如何变化.
主要方法:
- 利用纳米液体色谱-并联质谱 (纳米LC-MS/MS) 进行高通量蛋白质识别.
- 分析了从静止的Saccharomyces cerevisiae细胞中分离出来的80S核糖体的蛋白质组成.
- 研究了特定核糖体蛋白质缺陷对转化能力的影响.
主要成果:
- 在静止细胞中构成80S核糖体的79种核糖体蛋白 (RP) 中,确定了69种.
- 发现了一种实质性的核蛋白质组,包括444种辅助蛋白质,参与各种细胞功能,包括翻译,蛋白质折叠,新陈代谢,应激反应和降解.
- 在核糖体颗粒上观察到RP和辅助蛋白的显著固体几何学,根据生长条件和核糖体分数 (单体/多体) 变化.
- 证明了在特定的RP中存在的缺陷会导致静止期间的翻译能力受损.
结论:
- 静止的核糖体是一个复杂的分子机器,其蛋白质组成多样,超出了正规的核糖体蛋白质.
- 核糖体异质性及其相关蛋白质在静止期间调节转化活动和细胞功能方面发挥着关键作用.
- 了解核糖体的组成,可以了解细胞适应,衰老以及非增殖细胞中蛋白质合成的调节.
关键词:
细胞生物学 细胞生物学相关概念视频
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