在细胞微/纳米电穿孔中,焦尔加热和电流
Junjie Pan1, Xinyu Wang1, Chi-Ling Chiang1
1Department of Chemical and Biomolecular Engineering, The Ohio State University, Columbus, Ohio 43210, USA. lee.31@osu.edu.
Lab on a chip
|January 18, 2024
概括
局部化微/纳米电解 (MEP/NEP) 增强了细胞的转染. 这项研究揭示了电场,焦勒加热和电层大气如何影响MEP/NEP,并定义了电场的定义.
科学领域:
- 生物医学工程 生物医学工程
- 细胞生物学 细胞生物学
- 微流体学 微流体学
背景情况:
- 局部化微/纳米电穿孔 (MEP/NEP) 提供精确的细胞传染,具有很高的活力.
- 在MEP/NEP中的微/纳米通道控制电场,用于细胞操纵和货物交付.
- 现有的研究缺乏对MEP/NEP对转染的影响中电动学现象的全面分析.
研究的目的:
- 在MEP/NEP中研究电场,焦勒加热,电化 (EO) 和电泳 (EP) 的相互作用.
- 分析这些现象对单细胞转菌效率和活力的影响.
- 定义MEP/NEP系统的最佳操作参数.
主要方法:
- 使用微流体生物芯片进行MEP/NEP的单细胞水平分析.
- 不同的通道大小和应用于电压,以研究电动效应.
- 在转化过程中观察到泡形成,EO和EP动态.
主要成果:
- 焦尔加热导致在值电压以上的气泡形成.
- 电层透影响货物运输,降低了高电压时等离子体DNA的效率.
- 气泡形成和过度的EO速度会对货物交付产生负面影响.
结论:
- 了解电动学现象对于优化MEP/NEP至关重要.
- 定义了一个"电解区",以减轻泡形成和增强转染.
- 这项工作为设计高效和可行的MEP/NEP系统提供了洞察力.
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