水提取物的高通量转录组学检测了水处理过程中生物活动的减少
Jesse D Rogers1,2, Frederic D L Leusch3, Bryant Chambers1
1Center for Computational Toxicology and Exposure, Office of Research and Development, U.S. Environmental Protection Agency, Research Triangle Park, North Carolina 27711, United States.
Environmental science & technology
|January 18, 2024
概括
高通量转录组学揭示了废水和地表水污染物显著改变人类细胞中的基因表达. 先进的水处理有效地减少了这种生物活性,提高了水质监测的信心.
科学领域:
- 环境科学 环境科学
- 毒理学 毒理学 毒理学
- 分子生物学分子生物学
背景情况:
- 水源含有来自各种来源的化学污染物,对健康构成风险.
- 目前的化学分析在覆盖范围和成本方面存在局限性.
- 广泛覆盖的体外检测 (如转录组学) 可以评估更广泛的潜在影响.
研究的目的:
- 通过使用高通量转录学来评估现场衍生水提取物的生物活性.
- 评估先进水处理对降低生物活性的有效性.
主要方法:
- 使用了高通量转录组学.
- 乳腺癌细胞的MCF7被暴露在水中.
- 分析了细胞增殖,神经,雌激素和抗生素途径中的基因表达变化.
主要成果:
- 废水和地表水提取物诱导了基因表达的显著变化.
- 饮用水和回收水提取物,经过先进的处理后,显示生物活性降低.
- 再利用的水提取物诱导的基因表达变化比实验室空白物少.
结论:
- 转录学是监测水质的宝贵工具.
- 先进的水处理方法显著降低了回收水的生物活性.
- 基于生物测试的监测为评估水质提供了可靠的数据.
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