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Hsa_circ_0010882通过调节M1/M2巨细胞极化来促进肝细胞癌的进展

Ming Yang¹, Tao Yu¹, Li Han¹

¹Department of Medical Oncology, The Affiliated Xuzhou Municipal Hospital of Xuzhou Medical University, Xuzhou, China.

Journal of viral hepatitis

|January 18, 2024

概括

肝细胞癌 (HCC) 的进展与增加的circ_0010882有关,这促进了免疫抑制的M2巨细胞. 向circ_0010882可能通过改变巨细胞平衡来提供新的HCC治疗策略.

科学领域:

在瘤学瘤学.
免疫学免疫学免疫学
分子生物学分子生物学

背景情况:

肝细胞癌 (HCC) 是一种普遍存在的恶性瘤,其特点是免疫抑制性瘤微环境和患者的不良结果.
巨细胞两极分化,特别是M1/M2平衡,在调节瘤微环境和影响HCC进展方面发挥着至关重要的作用.

研究的目的:

这项研究旨在调查hsa_circ_0010882在M1/M2巨分化中在肝细胞癌进展的背景下发挥的作用.
这项研究旨在阐明circ_0010882影响巨细胞表型并影响HCC细胞行为的分子机制.

主要方法:

从接受肝切除术的患者中采集了配对的HCC组织样本 (n=125).
使用THP-1细胞诱导M1和M2巨细胞表型.
细胞实验评估了HCC细胞活力,迁移和与巨细胞和转染的HCC细胞共同培养后的入侵. 生物信息分析和双露西法酶记者测定被用来确认circ_0010882和miR-382.2之间的相互作用.

主要成果:

在HCC组织中,Circ_0010882的表达显著升高,与较短的整体存活率相关.
干扰circ_0010882促进了M1巨标记物 (TNF-α,iNOS),同时抑制了M2标记物 (Arg-1,CD206),表明从M2极化转移.
沉默circ_0010882增强了M1巨细胞介导的抑制HCC细胞活力,迁移和入侵,同时减少了M2巨细胞驱动的瘤促进. 确定了米R-382作为circ_0010882.2.的直接目标.

结论:

在HCC中,circ_0010882的表达上调,并与预后不佳有关.
沉默circ_0010882抑制HCC进展中的M2巨细胞极化,部分通过调节miR-382表达.
Circ_0010882具有作为诊断生物标志物和治疗点的潜力,可以改善HCC治疗策略和患者的治疗结果.

关键词:

hsa_circ_0010882 在线观看在线观看巨细胞是什么?巨细胞是什么?这就是为什么miR-382是MIR-382.预后预后预后这是一个渐进的过程.

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